FIGURE

Fig. 5

ID

ZDB-FIG-080417-25

Publication

Kotani et al., 2008 - misty somites, a maternal effect gene identified by transposon-mediated insertional mutagenesis in zebrafish that is essential for the somite boundary maintenance

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Fig. 5

The mys-deficiency impaired epithelialization of somitic cells. (A–D) Confocal images of embryos stained with the anti-γ-Tubulin antibody (red) and Phalloidin (green). Dorsal views of a wild type embryo (A, C) and an ATG-MO injected embryo (B, D) at 13 hpf (the 6-somite stage) (A, B) or at 16 hpf (the 14-somite stage) (C, D). Asterisks indicate the positions of the somite boundaries. In wild type, somitic cells adjacent to the boundaries show a columnar shape and apical localization of the centrosomes. In the ATG-MO injected embryo, the somitic cells do not show the columnar shape and the centrosomes are distributed randomly in the cytoplasm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-prrc1
Knockdown Reagent:	MO1-prrc1
Observed In:	epithelial structure maintenance somite epithelial cell
Stage Range:	5-9 somites to 14-19 somites

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 316(2), Kotani, T., and Kawakami, K., misty somites, a maternal effect gene identified by transposon-mediated insertional mutagenesis in zebrafish that is essential for the somite boundary maintenance, 383-396, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.