Fig. 2
In situ hybridization of a col15a1 antisense RNA probe on whole mount zebrafish embryos from 10 to 48 hpf. (A, B) at 10 hpf, col15a1 expression is exclusively restricted to the notochord. (C, D) at 15 hpf, expression decreases in notochord. An intense labelling remains in the caudal part (arrowhead). (E, F) at 24 hpf, col15a1 expression is vanished in notochord, except in the caudal part. A weak signal of hybridization is also observed in the head region and in otic vesicles (arrow). Insert shows the signal in the caudal notochord (arrow) and in the chordo-neural hinge (arrowhead). (G, H) at 36 hpf, a weak signal appears in pectoral fins. (I, J) at 48hpf, a more intense col15a1 expression is detected in brain, in otic vesicle and in pectoral fins. Magnification: A–D: x 350; E–J: x 300 |
Gene: | |
---|---|
Fish: | |
Anatomical Terms: | |
Stage Range: | Bud to Long-pec |
Reprinted from Developmental Biology, 316(1), Pagnon-Minot, A., Malbouyres, M., Haftek-Terreau, Z., Kim, H.R., Sasaki, T., Thisse, C., Thisse, B., Ingham, P.W., Ruggiero, F., and Le Guellec, D., Collagen XV, a novel factor in zebrafish notochord differentiation and muscle development, 21-35, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.