FIGURE

Fig. 2

ID

ZDB-FIG-080402-43

Publication

Lepage et al., 2008 - Characterization and comparative expression of zebrafish calpain system genes during early development

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Fig. 2

Temporal expression pattern and exon organization of four calpastatin splice variants. Semiquantitative reverse transcriptase (RT) -polymerase chain reaction using cast F1 and R1 primers was used to determine the relative abundance of four cast transcripts at 64-cell, sphere, shield, bud, and 1 day postfertilization (dpf). One representative no template control (no RT) is shown and β-actin was used as a loading control. Schematic representation of exon organization is shown for each of the four transcripts. cast F1 and R1 primers amplify exons 1-9 and F2 and R2 amplify exons 11-20 as indicated. cast F2 and R2 amplify a single product (see Fig. 3).

Expression Data

Gene:	cast
Fish:	WT
Anatomical Term:	whole organism
Stage Range:	64-cell to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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