Fig. 1

Morphology of the eye in Fgf19 MOs-injected embryos. A: Embryos were injected with control MO (a, b), Fgf19 MO1 (c, d), and Fgf19 MO2 (e, f). (a, b) In control MO-injected embryos, the eye developed normally. (c, d) Fgf19 MO1-injected embryos exhibited a significant reduction in the size of the lens and the retina at 72 hpf. (e, f) Fgf19 MO2-injected embryos also showed a significant reduction in the size of the eye, a failure of the choroid fissure to close, and the expansion of retinal tissue to the midline of the forebrain at 72 hpf. A failure of the choroid fissure to close, indicated by an arrowhead, was observed (c, e). A progressive expansion of retinal tissue to the midline of the forebrain, indicated by arrowheads, was also observed (d, f). (a, c, e) Lateral views with anterior to the left and dorsal to the top. (b, d, f) Ventral views with anterior to the top. B: (a) The coding region of Fgf19 was divided by two introns. Black boxes and black lines indicate exons and introns, respectively. Fgf19 MO1 anto the top. (b, d, f) Ventral views with anterior to the top. B: (a) The coding region of Fgf19 was divided by two intd Fgf19 MO2 indicate their target positions. Arrowheads indicate the positions of PCR primers for Fgf19 cDNA. (b) Fgf19 cDNA was amplified from wild-type or Fgf19 MO2-injected embryonic cDNA by RT-PCR using forward and reverse primers. ef1α cDNA was also amplified as a control. The cDNAs were analyzed by 1.5% agarose gel electrophoresis. After electrophoresis, the gel was stained with ethidium bromide.