Fig. 6
- ID
- ZDB-FIG-071228-20
- Publication
- Veldman et al., 2007 - Gene expression analysis of zebrafish retinal ganglion cells during optic nerve regeneration identifies KLF6a and KLF7a as important regulators of axon regeneration
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Knockdown of SOCS3a and SOCS3b or Sox11a and Sox11b has no effect on axon outgrowth in retinal explants. Morpholino-modified antisense oligonucleotides were delivered to RGCs in vivo via the sectioned optic nerve. Four days later, retinas were isolated, diced, and placed in explant culture for 4 days prior to analysis. Shown are representative images of axon outgrowth. Control morpholino-treated (A), SOCS3a and SOCS3b atg-targeting morpholino-treated (B), and Sox11a and Sox11b atg-targeting morpholino-treated explants (C) exhibit robust axon outgrowth after 4 days in culture. (D) Nerve Growth Index for explants from the indicated treatment groups: (-), no treatment (n = 23); C-MO, control morpholino (n = 34); 3a/b, SOCS3a/b-atg morpholino (n = 21); and 11a/b, Sox11a/b-atg morpholino (n = 68). Nerve Growth Index as determined by Landreth and Agranoff (1979) takes into account both axon length and density. Nerve Growth Index values were normalized to C-MO group that was defined as 100%. Error bars represent SEM. |
Reprinted from Developmental Biology, 312(2), Veldman, M.B., Bemben, M.A., Thompson, R.C., and Goldman, D., Gene expression analysis of zebrafish retinal ganglion cells during optic nerve regeneration identifies KLF6a and KLF7a as important regulators of axon regeneration, 596-612, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.