FIGURE

Fig. S4

ID

ZDB-FIG-071204-11

Publication

Jazwinska et al., 2007 - Activin-betaA Signaling Is Required for Zebrafish Fin Regeneration

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Fig. S4

ActβA Signaling Is Required for Blastemal Proliferation. (A–D) Longitudinal sections of fin rays at 3.5 dpa. In control fins, the blastema (arrowheads) displays a massive proliferation as visualized by BrdU incorporation in green (A) and only sparse mesenchymal cells labeled by TUNEL cell-death assay in green (C). Fins regenerating in normal conditions for 3 days and then transferred to water with Alk-i for the next 12 hr display a reduction of blastemal proliferation (B). No enhancement in mesenchymal cell death is present after the drug shift (D). Cell death is detected in the outer layers of the epidermis. (E and F) Quantification of BrdU-positive cells in the epidermis and in the mesenchyme after inhibitor treatment for 24 hpa starting immediately after amputation (E) and after the drug shift (3 days at normal conditions, followed by the next 6, 12 or 24 hr with Alk4-i (F). The values are presented as a percentage of the control samples, which were set as 100%. Error bars represent the SEM. n = 10 (two or three representative sections from four fins were analyzed). *p < 0.01 indicates a significant difference between inhibitor-treated fins and the control.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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