FIGURE

Fig. 5

ID

ZDB-FIG-071112-6

Publication

Kosaka et al., 2007 - Spatiotemporal localization of germ plasm RNAs during zebrafish oogenesis

Other Figures

All Figure Page

Back to All Figure Page

Fig. 5

The 3′ UTRs of vasa, nanos1 and dazl RNAs govern localization during oogenesis and early embryogenesis. In situ hybridization of GFP mRNA fused with vasa, nanos1, dazl, or SV40 3′ UTRs expressed in transgenic zebrafish oocytes (stages I and III) and fluorescein in situ hybridization in 4-cell embryos. GFP-nanos1 mRNA was expressed by zpc promoter (Onichtchouk et al., 2003), whereas other mRNAs were expressed by EF1α promoter. In stage I oocytes, in situ hybridization (RNA) was performed on sections counter-stained with Mitotracker (Mitochondria). Oocyte sections were cut at a thickness of 10 μm. Scale bars, 50 μm. Lateral and animal views of the 4-cell embryo are shown.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Mechanisms of Development, 124(4), Kosaka, K., Kawakami, K., Sakamoto, H., and Inoue, K., Spatiotemporal localization of germ plasm RNAs during zebrafish oogenesis, 279-289, Copyright (2007) with permission from Elsevier. Full text @ Mech. Dev.