Fig. 9

N-cadherin expression during epiboly and segmentation. (A) RT–PCR products. (B–L) In situ hybridizations with the N-cadherin probe. (B) Presumptive dorsal view; inset shows magnified view of yolk syncytial layer. (C) Dorsal view; inset shows magnified side view of deep cells. (D) Dorsal view. (E) Medial sagittal section, dorsal is right; arrowheads demarcate specific layers of the blastoderm. (F) Magnified view of boxed region in panel E, arrow indicates radial gradient of N-cadherin transcripts. (G) Dorsal view: left side, deep optical cross-section; right side, superficial view of same embryo. (H) Side view of panel G, deep optical cross-section, dorsal is right. (I) Flat mount dorsal view, lines indicate levels corresponding to transverse cross-sections J–L. (J) Head section, (K) trunk section, and (L) tailbud section. For panels J–L, black brackets demarcate neural plate, white brackets demarcate paraxial mesoderm, black arrowheads indicate edge of neural plate, white arrowheads indicate edge of paraxial mesoderm, and arrow indicates edge of lateral plate mesoderm. (M) Transverse cross section of a same stage embryo probed with papc. Abbreviations: dorsal forerunner cells, dfc; epiblast, epi; enveloping layer, evl; hypoblast, hyp; interior deep cells, idc; shield, sh; notochord, n; yolk syncytial layer, ysl.