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Fig. 2

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ZDB-FIG-071005-31

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Kozlowski et al., 2005 - The zebrafish dog-eared mutation disrupts eya1, a gene required for cell survival and differentiation in the inner ear and lateral line

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Fig. 2

Morpholino-mediated phenocopy of dog-eared. Lateral views of control (A, C, and E) or eya1MO-injected (B, D, and F) embryos at 72 hpf. (A and B) Otocyst morphology as viewed by DIC microscopy. (C and D) Otocyst cell death visualized by acridine orange staining of live embryos shown in panels A and B. (E and F) Visualization, using the TUNEL assay, of apoptotic cell death in the otocyst of embryos shown in panels A and B. Anterior to left and dorsal to top. Scale bar, 100 μm. (G and H) RT-PCR analysis of eya1 mRNA processing at the exon10–exon11 junction in eya1MO-injected, or mismatched morpholino (mutMO)-injected, or buffer-injected embryos at 24 hpf (G) and 48 hpf (H). Products of reactions lacking reverse transcriptase (RT) or containing genomic DNA (gDNA) are also shown. The white arrow indicates the position of the expected 605-bp fragment amplified from correctly spliced mRNA. Each lane represents five pooled embryos.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 277(1), Kozlowski, D.J., Whitfield, T.T., Hukriede, N.A., Lam, W.K., and Weinberg, E.S., The zebrafish dog-eared mutation disrupts eya1, a gene required for cell survival and differentiation in the inner ear and lateral line, 27-41, Copyright (2005) with permission from Elsevier. Full text @ Dev. Biol.