Zebrafish nz171 homozygous mutants lack runx1 and runx3 expression, but retain normal expression of cbfb. (A-C) runx1 expression and morphology of nz171 mutants. (A) Loss of runx1 expression in the PLM (posterior lateral-plate mesoderm; arrows) of an nz171 mutant embryo (mut) compared with wild-type sibling (WT); posterior views of 10-somite embryos. (B,C) Morphology of nz171 mutant (mut) embryos compared with siblings (WT) at the stages indicated (B, dorsal; C, anterior to the left). (D-I) Expression of runx1, runx3, and cbfb in wild-type and nz171 mutant embryos (14-somite embryos, anterior to the left). In nz171 mutants, expression of runx1 is lost in the ICM (intermediate cell mass; icm), ALM (anterior lateral-plate mesoderm; *) and olfactory placode (op) but retained in a subset of the Rohon-Beard (RB) mechanosensory neurons (rb) (G compared with D, wild type). nz171 mutants lack all runx3 expression (H compared with E, wild type). cbfb expression is normal in nz171 mutants (I compared with F, wild type).
|
