FIGURE

Fig. 2

ID

ZDB-FIG-070613-3

Publication

Shin et al., 2007 - Notch signaling regulates neural precursor allocation and binary neuronal fate decisions in zebrafish

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Fig. 2

Loss of Notch signaling causes formation of excess PMNs and a deficit of KA' interneurons. (A-D) Lateral views of the spinal cord at the 6- to 12-somite region of zebrafish embryos, with anterior to left and dorsal at the top. (A) A subset of PMNs (CaP and VaP) and Rohon-Beard neurons of Tg(olig2:egfp) embryos expressed isl2 in the ventral spinal cord (black bracket) and dorsal spinal cord (white bracket) at 20 hpf, respectively. (B) In Tg(olig2:egfp) embryos, KA' interneurons (arrowheads) were detected by anti-GABA antibody (red) within EGFP⁺ cells at 25 hpf. EGFP^- GABA⁺ cells (arrows) in ventral spinal cord are KA'' interneurons (Park et al., 2004). (C) In mib^-/-;Tg(olig2:egfp) embryos, isl2⁺ PMNs formed as clusters in each hemisegment in the ventral spinal cord (black bracket). (D) mib^-/-;Tg(olig2:egfp) embryos did not produce EGFP⁺ GABA⁺ KA' interneurons, although EGFP^- GABA⁺ KA'' interneurons were still present (arrows). (E) Mean of the number of PMNs (gray bar) and KA' interneurons (black bar) per hemisegment in Tg(olig2:egfp) and mib^-/-;Tg(olig2:egfp) embryos (n=7). Error bars represent s.e.m. Scale bar: 50 µm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mib1^ta52b/ta52b; vu12Tg
Observed In:	spinal cord CNS interneuron spinal cord primary motor neuron spinal cord motor neuron differentiation ventral spinal cord interneuron differentiation
Stage Range:	20-25 somites to Prim-5

Phenotype Detail

Acknowledgments

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