FIGURE

Fig. 1

ID

ZDB-FIG-070502-28

Publication

Hagos et al., 2007 - Time-dependent patterning of the mesoderm and endoderm by Nodal signals in zebrafish

Other Figures

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Fig. 1

Treatment with 800 μM SB-431542 or 50 μM SB-505124 at MBT prevents formation of mesoderm and endoderm. (A-F) Images of live embryos at 24hpf treated at 2.75 h with DMSO (A-C), SB-431542 (D-F; J-S), or SB-505124 (G-I). Embryos treated with SB-431542 (D-F) or SB-505124 (G-I) lack derivatives of the mesoderm and endoderm in the head and trunk, display severe cyclopia and lack Kupffer's vesicle. (J-P) Images of embryos treated with DMSO (J-N) or SB-431542 at MBT (O-S) and processed to reveal expression of markers for derivatives of dorsal mesoderm (ntl: J, O; flh: L, Q), paraxial mesoderm (myoD: K, P), intermediate mesoderm (pax2.1: M, R), and ventral neurectoderm (shhb: N, S). Dorsal views of embryos fixed at 10 h (J, L, N, P, Q, S)or 14 h (K, M, P, R). Arrowhead in (C) is the Kupffer's vesicle.

Expression Data

Genes:	myod1 noto pax2a shhb tbxta
Fish:	WIK
Condition:	chemical treatment: pharmaceutical
Anatomical Terms:	axial mesoderm intermediate mesoderm neuroectoderm notochord paraxial mesoderm somite (all 6)
Stage Range:	Bud to 10-13 somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Dev. Biol.