Fig. 7
Locally Applied Ca2+ Chelators Induce Movement of Lateral Mesodermal Cells in an Alk8-Independent Fashion and without Affecting Dorsoventral Patterning (A)–(D) Live embryos with a fluorescently labeled group of lateral mesodermal cells at the onset of gastrulation (shield) and at a late gastrula stage (90% epiboly). (A, E, and F) bmp2b morphants with dorsolateral BAPTA beads. (B) bmp2b morphant with ventrolateral BAPTA bead. (C) bmp2b morphant after dorsolateral injection of EGTA (arrow). (D) alk8 morphant with dorsolateral BAPTA bead. (E and F) Vegetal views of embryos at 80% epiboly stage; (E) anti-phos Smad1/5/8 immunostaining; (F) tbx24 in situ hybridization. Implanted beads are indicated by arrowheads. |