amotl2 expression and regulation by Fgf signal in zebrafish embryos. (A-O) amotl2 expression in wild type was detected by whole-mount in situ hybridization at two-cell (A), 1k-cell (B), sphere (C), shield (D), 70% epiboly (E), two-somite (F,G), ten-somite (H,I), 18-somite (J), 24 hpf (K,I) and 36 hpf (M-O) stages. (A-E) Lateral views with dorsal to the right; (F-I,O) dorsal views with anterior to the left; (J-N) lateral views with anterior to the left. (F and G; H and I) The anterior (F,H) and posterior (G,I) regions for the same embryo, respectively. (L) Higher magnification of the region boxed in K. (N) Higher magnification of the region boxed in M. (O) Head of the embryo in M. (P-W) amotl2 expression in embryos injected with 14 pg fgf8 mRNA (Q-S) or treated with Fgfr signaling inhibitor SU5402 (U-W). (P-S) Animal pole views at the shield stage with dorsal to the right. (T-W) Lateral views at the 60% epiboly stage with dorsal to the right. fgf8 mRNA was injected into a one-cell embryo (Q) or a single cell located in the animal pole at the 32-cell stage (R) or the 64-cell stage (S). amotl2 expression was strongly induced in the circled area following single-cell injection at multi-cell stages (R,S). SU5402 treatment started at the sphere stage. av, axial vasculature; dn, dorsal neuron; hbr, hindbrain rhombomere; hg: hatching gland; iv, intersegmental vessel; llp, lateral line primordium; n, neuron; p, polster; s, somite; t, tectum; tn, telencephalon; tp, trigeminal placodes; vn, ventral neuron.
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