Timecourse of anterior trunk pigment pattern formation in WT and cho mutants during period of collar formation. Lateral views of WT (A,C,E) and cho mutant (B,D,F) embryos are shown. Melanophores were seen on the lateral pathway in cho mutants and WT siblings at 34 hpf (A,B), and were thereafter absent from WT, but accumulated steadily in cho mutants (C-F). Note too the change in collar melanophore morphology between 58 hpf, when their stellate morphology correlated with migratory activity, and 74 hpf, when their more flattened shape reflects a more static phase. Scale bar: 50 μ;m. (G) Total number (means±s.e.) of melanophores on lateral pathway of somites 1-5 throughout collar formation.
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