FIGURE

Fig. 4

ID

ZDB-FIG-060901-4

Publication

Li et al., 2006 - The transcription factor ZBP-89 controls generation of the hematopoietic lineage in zebrafish and mouse embryonic stem cells

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Fig. 4

Rescue of zbp-89 morphants by zebrafish scl sense RNA. (A-F) Gata1-driven GFP in 22 hpf transgenic (gata1:EGFP) zebrafish embryos that were untreated (A,D), injected with atgMO only (B,E), or injected with atgMO plus scl RNA (C,F) at the one- to two-cell stage. (A-C) Single 22 hpf embryos examined under a fluorescent microscope revealing gata1 expression (red arrows) in the ICM of the untreated (A) and scl-rescued (C) embryos, but not in the atgMO only-injected (B) embryos. (D-F) Bright-field low magnification images of the respective untreated and treated Tg gata1:EGFP embryos at 48 hpf. Short arrows in E point to pericardial edema in the zbp-89 morphants.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-znf148 la781Tg + MO1-znf148
Knockdown Reagent:	MO1-znf148
Observed In:	blood nucleate erythrocyte heart contraction pericardium
Stage Range:	26+ somites to Long-pec

Phenotype Detail

Acknowledgments

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