FIGURE

Fig. 4

ID

ZDB-FIG-060809-4

Publication

Mercader et al., 2006 - Prdm1 acts downstream of a sequential RA, Wnt and Fgf signaling cascade during zebrafish forelimb induction

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Fig. 4

Mosaic analysis in raldh2 and prdm1 morphants. (A) Dorsal view of a three-day-old raldh2 morphant embryo revealing rescued pectoral fin outgrowth on the right side (arrow). (A') Dark-field image of the same embryo, showing transplanted GFP-positive cells labeled in green. (A'') Merged bright-field and dark-field images showing green wild-type cells localizing to the anterior somite region. (B-B'') Lateral views of the same MOraldh2 mosaic embryo as in A. Dotted lines in B' indicate somite boundaries. Note strong GFP-expression in somites 1 to 3. (C-D'') Dorsal and lateral views of an MOprdm1 embryo, where transplanted wild-type cells contribute to anterior somites but do not rescue fin outgrowth. (C'',D'') Merged bright and dark field pictures showing GFP-positive wild-type cells incorporated into the left fin. Dotted lines in D' indicate somite boundaries. Asterisks mark the missing pectoral fin. s, somite.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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