Fig. 4

lama 1 knockdown phenotype in zebrafish. A, an overall view of lama1-morphants obtained with MO1- (top) or MO2- oligomers (middle), and control (bottom) embryos at 72-hpf. Enlarged image of a head is provided on the right. Defects in body length, axis curvature and eye structure (irregular pupil and a lack of lens) are easily detectable in lama1 morphants. B, transverse sections at the eye level of control (top row) and lama1-morphant (bottom row) embryos at 24-, 32-, 48- and 72-hpf are presented. An obvious lens degeneration is first notable in 48-hpf morphant eyes. At 72-hpf, small eyes with missing lens and thickened cornea were observed in the morpholino-injected embryos. retina (r), optic nerve (on), lens (le) and cornea (c) are shown. Black arrows in 48- and 72-hpf eyes indicate to the products of lens degeneration.