FIGURE

Fig. 3

ID

ZDB-FIG-060221-2

Publication

Link et al., 2006 - Proteomics of early zebrafish embryos

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Fig. 3

Improved Western blotting results. Embryos at high stage (3 1/3 hpf), 50% epiboly (5 1/4 hpf), 70% epiboly (7 hpf) and tailbud stage (10 hpf) were deyolked, separated by SDS-gel electrophoresis and Coomassie stained (A) or blotted and immunodetected with antibodies against Tubulin (55 kD) and Moesin (78/80 kD apparent molecular weight) (B). Note that total protein amount was lower in deyolked samples, therefore more embryos could be loaded per lane: 1 embryo with yolk (Y), 15 embryos deyolked (D), 15 embryos deyolked and washed twice (W). Consequently, signal intensities of cellular proteins were increased.

Expression Data

Gene:	msnb
Fish:	WT
Anatomical Term:	whole organism
Stage Range:	1k-cell to Bud

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Dev. Biol.