FIGURE

Fig. 2

ID

ZDB-FIG-060118-3

Publication

Pollard et al., 2006 - Essential and overlapping roles for laminin alpha chains in notochord and blood vessel formation

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Fig. 2

Morphological and molecular analysis of bal, gup and sly mutant embryos. Lateral views show wild-type (WT) (A, G), bal (D, K), gup (H) and sly (L) embryos at 3 day post fertilisation. Staining with a polyclonal antibody generated against mouse laminin 1 (α1β1γ1) detects expression pattern in 24 hpf WT (B) embryos. Laminin immunoreactivity in 24 hpf bal mutant embryos (E) is at wild-type levels within the posterior notochord (arrowhead), but severely reduced immunoreactivity is seen in the non-differentiated anterior notochord, and intersomitic boundaries. In 24 hpf gup (I) and sly (M) embryos, laminin 1 immunoreactivity is abolished. The developing vasculature is marked by expression of fli-1 mRNA in 24 hpf WT (C) and bal (F), gup (J) and sly (N) mutant embryos.

Expression Data

Genes:	fli1 lama1
Fish:	WT lama1^m190/m190 (AB) lamb1a^m189/m189 (AB) lamc1^m466/m466 (AB)
Anatomical Terms:	blood vasculature intersegmental vessel unspecified
Stage Range:	14-19 somites to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	lama1^m190/m190 (AB) lamb1a^m189/m189 (AB) lamc1^m466/m466 (AB)
Observed In:	intersegmental vessel notochord notochord morphogenesis post-vent region vertical myoseptum whole organism
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 289(1), Pollard, S.M., Parsons, M.J., Kamei, M., Kettleborough, R.N., Thomas, K.A., Pham, V.N., Bae, M.K., Scott, A., Weinstein, B.M., and Stemple, D.L., Essential and overlapping roles for laminin alpha chains in notochord and blood vessel formation, 64-76, Copyright (2006) with permission from Elsevier. Full text @ Dev. Biol.