Expression patterns of cell type and region-specific genes during development of mutants affecting dorsal fates. (A,B) Expression of gsc and ntl RNA in wild-type (wt) (A) and bozm168 (B) mutant embryos at the bud stage (9.5 hpf). pp, gsc expression domain in prechordal plate; nk, gsc expression domain in neural keel; nt, ntl expression domain in chordamesoderm; bm, ntl expression domain in the blastoderm margin. (C,D) Expression pattern of hlx1 RNA in wild-type (C) and bozm168 mutant embryos at 30 hpf; de, mb, expression domain in diencephalon and midbrain, respectively. (E,F) Expression of shh RNA in wild-type (E) and trim209 mutant embryo at 30 hpf. ey, eye; fb, forebrain; hb, hindbrain; mb, midbrain. Scale bar, 0.1 mm.
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