FIGURE

Fig. 3

ID

ZDB-FIG-051220-40

Publication

Kohli et al., 2005 - Potential targets of transforming growth factor-beta1 during inhibition of oocyte maturation in zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 3

TGF-β1 inhibits mRNA expression of 20β-HSD. (A) Follicles were treated with control medium or 10 ng/ml of TGF-β1 for 2, 6, 12 and 24 hours. (B) Follicles were treated with different concentrations (0, 0.1,1 and 10 ng/ml) of TGF-β1 for 18 hours. (C) Follicles were treated with control medium, hCG (100 IU/ml), TGF-β1 (10 ng/ml), or hCG+ TGF-β1 for 18 hours. Total RNA was extracted and subjected to RT-PCR using primers for 20β-HSD and GAPDH. Each value represents the mean ± SEM of three replicates in one representative RT-PCR reaction. 20β-HSD mRNA levels are expressed as percent of control after normalized with the GAPHD levels. Different letters above the bars denote statistical significance (P < 0.05). *, P < 0.05 vs. control. The insets show representative ethidium bromide stained gels. GAPDH gels are the same for Figs. 4-7.

Expression Data

Gene:	cbr1l
Fish:	WT
Condition:	chemical treatment: pharmaceutical, organ culture: ovarian follicle
Anatomical Term:	ovarian follicle
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Reprod. Biol. Endocrinol.