FIGURE

Fig. 2

ID

ZDB-FIG-051118-4

Publication

Shepard et al., 2005 - A zebrafish bmyb mutation causes genome instability and increased cancer susceptibility

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Fig. 2

bmyb is the crb gene and may act through regulation of cyclin B. (A) crb positional cloning schematic with an enlarged view of the crb locus that depicts the BAC genomic clones identified in a chromosomal walk in analysis of 3,068 meioses from diploid mutants. The number of recombination events identified from the distal side (squares) and proximal side (circles) are indicated. (B) Schematic representation of the mutation in bmyb found in the crb mutants. Splice donor mutation (GT to GG) occurred between exon 13 and exon 14 (just after amino acid 591) causing a frameshift in the protein. (C) Phenotypic rescue (assessed by pH3 staining) of 24-hpf crb embryos by injection of bmyb mRNA and phenocopy of crb by injection of bmyb morpholinos. (D) Whole-mount embryo mRNA in situ hybridization analysis for cyclin B1 and cyclin E2. (E) Phenotypic rescue of 24 hpf crb embryos by injection of cyclin B1 mRNA.

Expression Data

Genes:	ccnb1 ccne2
Fish:	WT mybl2b^cz226/cz226 (WIK)
Anatomical Term:	unspecified
Stage Range:	5-9 somites to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-mybl2b + MO2-mybl2b
Knockdown Reagents:	MO1-mybl2b MO2-mybl2b
Observed In:	cell population proliferation head whole organism
Stage:	Prim-5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA