FIGURE

Fig. 3

ID

ZDB-FIG-050120-7

Publication

Little et al., 2004 - Twisted gastrulation promotes BMP signaling in zebrafish dorsal-ventral axial patterning

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Fig. 3

Interaction between sub-dorsalizing tsg1 knockdown and swirl (bmp2b) heterozygotes. All embryos were injected with 16 ng tsg1 MO. At 24 hpf, an injected wild-type embryo was not dorsalized (A), whereas injected swirl (bmp2b) heterozygous siblings were dorsalized. The extent of dorsalization was categorized as follows: (B) weak, class 1 (arrowhead indicates partial loss of the ventral tail fin); (C) moderate, class 3; and (D) strong, class 4. (E-J) Whole-mount in situ hybridization of myod, pax2a and krox20 in 16 ng tsg1 MO1 injected embryos at the eight-somite stage. Injected wild-type embryos were not affected (E,F), whereas heterozygotes displayed a range of dorsalizations (G,H, moderate; I, strong; J, stronger). (F,H) More posterior views of the same embryos shown in E,G, respectively. (J) The somites extend around the circumference, which is typical of a strong, class 4 dorsalization.

Expression Data

Genes:	egr2b myod1 pax2a
Fish:	WT + MO1-twsg1b bmp2b^{tc300a/tc300a} + MO1-twsg1b (TU)
Knockdown Reagent:	MO1-twsg1b
Anatomical Terms:	mesoderm midbrain hindbrain boundary neural rod presumptive rhombomere 3 presumptive rhombomere 5
Stage:	5-9 somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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