FIGURE

Fig. 7

ID

ZDB-FIG-041108-7

Publication

Hans et al., 2004 - Pax8 and Pax2a function synergistically in otic specification, downstream of the Foxi1 and Dlx3b transcription factors

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Fig. 7

Foxi1 and Dlx3b mediate convergent pathways of otic development. In foxi1^- mutants, otic tissue is reduced (B,G,L,O,T) compared with wild-type embryos (A,F,K,N,S) assessed both by morphology (A,B,F,G) and markers, including Dlx3b (K,L), cldna (N,O) and pax2a (S,T). foxi1^-;pax2a^– double mutants show no morphological sign of an otic vesicle (C,H) and no expression of Dlx3b (M) or pax2a (U), although some residual ‘otic’ cells can be detected with cldna (P). Injection of dlx3b-MO into wild-type embryos leads to reduction of overall ear size (D,I) that is preceded by reduced expression of cldna (Q) and pax2a (V). In foxi1^- mutant embryos depleted of Dlx3b, all otic specification is absent as indicated by morphology (E,J) and transcription of cldna (R) and pax2a (W). (A-M) Side views, anterior towards the left, dorsal towards the top; (N-W) dorsal views, anterior towards the top. Scale bar: 200 μm for A-E; 75 μm for F-J; 60 μm for K-M; 100 μm for N-W. n.d., not done.

Expression Data

Genes:	cldna dlx3b pax2a
Fish:	WT foxi1^em1/em1 WT + MO1-dlx3b foxi1^em1/em1 + MO1-dlx3b
Knockdown Reagent:	MO1-dlx3b
Anatomical Term:	otic vesicle
Stage:	26+ somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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