CRISPR

CRISPR3-nr2f1b

ID
ZDB-CRISPR-241031-4
Name
CRISPR3-nr2f1b
Previous Names
None
Target
Sequence
5' - CGCGTGGAGAGATCCGCAGG - 3'
ZFIN BLAST NCBI BLAST Ensembl UCSC BLAT
Disclaimer
Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.
Note
The PAM site was"AGG" at the 3' end.
Genome Resources
None
Target Location
Constructs
No data available
Genomic Features
Genomic Feature Affected Genomic Regions
sud22 nr2f1b
Expression
Gene expression in Wild Types + CRISPR3-nr2f1b
No data available
Phenotype
Phenotype resulting from CRISPR3-nr2f1b
No data available
Phenotype of all Fish created by or utilizing CRISPR3-nr2f1b
Phenotype Fish Conditions Figures
preoptic area nkx2.1 expression increased amount, abnormal nr2f1bsud22/sud22 standard conditions Fig. 7 with image from Chowdhury et al., 2024
optic tectum apoptotic process increased frequency, abnormal nr2f1bsud22/sud22 standard conditions Fig. 5 with image from Chowdhury et al., 2024
eye decreased size, abnormal nr2f1bsud22/sud22 standard conditions Fig. 4 with image from Chowdhury et al., 2024
eye pax6a expression decreased amount, abnormal nr2f1bsud22/sud22 standard conditions Fig. 7 with image from Chowdhury et al., 2024
brain decreased size, abnormal nr2f1bsud22/sud22 standard conditions Fig. 4 with image from Chowdhury et al., 2024
preoptic area dlx2a expression increased amount, abnormal nr2f1bsud22/sud22 standard conditions Fig. 7 with image from Chowdhury et al., 2024
telencephalon anterior side vax2 expression increased amount, abnormal nr2f1bsud22/sud22 standard conditions Fig. 7 with image from Chowdhury et al., 2024
forebrain pax6a expression decreased amount, abnormal nr2f1bsud22/sud22 standard conditions Fig. 7 with image from Chowdhury et al., 2024
eye apoptotic process increased frequency, abnormal nr2f1bsud22/sud22 standard conditions Fig. 5 with image from Chowdhury et al., 2024
optic stalk pax2a expression decreased amount, abnormal nr2f1bsud22/sud22 standard conditions Fig. 7 with image from Chowdhury et al., 2024
hindbrain apoptotic process increased frequency, abnormal nr2f1bsud22/sud22 standard conditions Fig. 5 with image from Chowdhury et al., 2024
telencephalon anterior side vax2 expression increased distribution, abnormal nr2f1bsud22/sud22 standard conditions Fig. 7 with image from Chowdhury et al., 2024
whole organism dead, abnormal nr2f1bsud22/sud22 standard conditions Fig. 3 with image from Chowdhury et al., 2024
preoptic area vax2 expression mislocalised, abnormal nr2f1bsud22/sud22 standard conditions Fig. 7 with image from Chowdhury et al., 2024
Citations
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