CRISPR

CRISPR5-vtg2

ID
ZDB-CRISPR-240911-2
Name
CRISPR5-vtg2
Previous Names
None
Target
Sequence
5' - ACGGTTCTCTCAGCAGA - 3'
ZFIN BLAST NCBI BLAST Ensembl
Disclaimer
Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.
Note
The PAM site was "GGA" at the 5' end.
Genome Resources
None
Target Location
Constructs
No data available
Genomic Features
Genomic Feature Affected Genomic Regions
inr103 vtg2
Expression
Gene expression in Wild Types + CRISPR5-vtg2
No data available
Phenotype
Phenotype resulting from CRISPR5-vtg2
No data available
Phenotype of all Fish created by or utilizing CRISPR5-vtg2
Phenotype Fish Conditions Figures
whole organism vtg1 expression decreased amount, abnormal vtg2inr103/inr103 standard conditions Figure 4 with image from Yilmaz et al., 2023
hatching increased process quality, abnormal vtg2inr103/inr103 standard conditions Figure 5 with image from Yilmaz et al., 2023
whole organism vtg3 expression decreased amount, abnormal vtg2inr103/inr103 standard conditions Figure 4 with image from Yilmaz et al., 2023
early embryonic cell cell division decreased process quality, abnormal vtg2inr103/inr103 standard conditions Figure 7 with image from Yilmaz et al., 2023
pericardium edematous, abnormal vtg2inr103/inr103 standard conditions Figure 7 with image from Yilmaz et al., 2023
post-vent region curved, abnormal vtg2inr103/inr103 standard conditions Figure 7 with image from Yilmaz et al., 2023
coelom edematous, abnormal vtg2inr103/inr103 standard conditions Figure 7 with image from Yilmaz et al., 2023
whole organism vtg2 expression decreased amount, abnormal vtg2inr103/inr103 standard conditions Figure 4 with image from Yilmaz et al., 2023
whole organism vtg4 expression decreased amount, abnormal vtg2inr103/inr103 standard conditions Figure 4 with image from Yilmaz et al., 2023
whole organism viability, abnormal vtg2inr103/inr103 standard conditions Figure 6 with image from Yilmaz et al., 2023
yolk inserted into perivitelline fluid, abnormal vtg2inr103/inr103 standard conditions Figure 7 with image from Yilmaz et al., 2023
whole organism vtg6 expression decreased amount, abnormal vtg2inr103/inr103 standard conditions Figure 4 with image from Yilmaz et al., 2023
female organism liver vtg5 expression increased amount, abnormal vtg2inr103/+ standard conditions Figure 4 with image from Yilmaz et al., 2023
fertilized egg decreased amount, abnormal vtg2inr103/+ standard conditions Figure 5 with image from Yilmaz et al., 2023
female organism liver vtg3 expression increased amount, abnormal vtg2inr103/+ standard conditions Figure 4 with image from Yilmaz et al., 2023
female organism liver vtg2 expression decreased amount, abnormal vtg2inr103/+ standard conditions Figure 4 with image from Yilmaz et al., 2023
Citations
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