CRISPR

CRISPR8-capn3b

ID

ZDB-CRISPR-240416-13

Name

CRISPR8-capn3b

Previous Names

None

Target

capn3b (1)

Sequence

5' - GGAATGGAGAAAAGTTGGGT - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
hsi15	capn3b

Expression

Gene expression in Wild Types + CRISPR8-capn3b

No data available

Phenotype

Phenotype resulting from CRISPR8-capn3b

No data available

Phenotype of all Fish created by or utilizing CRISPR8-capn3b

Phenotype	Fish	Conditions	Figures
trunk musculature hspb11 expression increased amount, abnormal	mitfa^w2/w2; capn3b^hsi15/hsi15; mpv17^a9/a9	chemical treatment by environment: azinphos-methyl	Figure 6 from Prykhozhij et al., 2023
trunk skeletal muscle cell damaged, exacerbated	mitfa^w2/w2; capn3b^hsi15/hsi15; mpv17^a9/a9	chemical treatment by environment: azinphos-methyl	Figure 6 from Prykhozhij et al., 2023
trunk skeletal muscle cell damaged, abnormal	mitfa^w2/w2; capn3b^hsi15/hsi15; mpv17^a9/a9	control	Figure 6 from Prykhozhij et al., 2023
whole organism capn3b expression absent, abnormal	mitfa^w2/w2; capn3b^hsi15/hsi15; mpv17^a9/a9	standard conditions	Figure 1 from Prykhozhij et al., 2023
post-vent region musculature system capn3b expression absent, abnormal	mitfa^w2/w2; capn3b^hsi15/hsi15; mpv17^a9/a9	standard conditions	Figure 2 from Prykhozhij et al., 2023
post-vent region musculature system hspb11 expression increased amount, abnormal	mitfa^w2/w2; capn3b^hsi15/hsi15; mpv17^a9/a9	chemical treatment by environment: azinphos-methyl	Figure 6 from Prykhozhij et al., 2023
trunk musculature capn3b expression absent, abnormal	mitfa^w2/w2; capn3b^hsi15/hsi15; mpv17^a9/a9	standard conditions	Figure 2 from Prykhozhij et al., 2023
pharyngeal arch 3-7 capn3b expression absent, abnormal	mitfa^w2/w2; capn3b^hsi15/hsi15; mpv17^a9/a9	standard conditions	Figure 2 from Prykhozhij et al., 2023
trunk skeletal muscle cell disorganized, abnormal	mitfa^w2/w2; capn3b^hsi15/hsi15; mpv17^a9/a9	chemical treatment by environment: methyl cellulose	Figure 4 from Prykhozhij et al., 2023

Citations