CRISPR

CRISPR3-nphs1

ID

ZDB-CRISPR-231208-2

Name

CRISPR3-nphs1

Previous Names

None

Target

nphs1 (1)

Sequence

5' - GGGCCTCAGGGGCCGTGCAGT - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "GGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
mss102	nphs1
mss103	nphs1

Expression

Gene expression in Wild Types + CRISPR3-nphs1

No data available

Phenotype

Phenotype resulting from CRISPR3-nphs1

No data available

Phenotype of all Fish created by or utilizing CRISPR3-nphs1

Phenotype	Fish	Conditions	Figures
mesonephric podocyte podocyte foot increased width, abnormal	nphs1^{mss102/mss102} (AB)	standard conditions	FIGURE 3 from Lee et al., 2022
heart edematous, abnormal	nphs1^{mss102/mss102} (AB)	standard conditions	FIGURE 1 from Lee et al., 2022
mesonephric podocyte mesonephric podocyte development decreased process quality, abnormal	nphs1^{mss102/mss102} (AB)	standard conditions	FIGURE 3 from Lee et al., 2022
pronephric podocyte podocyte development decreased process quality, abnormal	nphs1^{mss102/mss102} (AB)	standard conditions	FIGURE 3 from Lee et al., 2022
mesonephric podocyte lacks all parts of type mesonephric podocyte slit diaphragm, abnormal	nphs1^{mss102/mss102} (AB)	standard conditions	FIGURE 3 from Lee et al., 2022
pronephric podocyte podocyte foot increased width, abnormal	nphs1^{mss102/mss102} (AB)	standard conditions	FIGURE 3 from Lee et al., 2022
orbital region edematous, abnormal	nphs1^{mss102/mss102} (AB)	standard conditions	FIGURE 1 from Lee et al., 2022
whole organism edematous, abnormal	nphs1^{mss102/mss102} (AB)	standard conditions	FIGURE 1 from Lee et al., 2022
pronephric podocyte lacks all parts of type pronephric podocyte slit diaphragm, abnormal	nphs1^{mss102/mss102} (AB)	standard conditions	FIGURE 3 from Lee et al., 2022
mesonephric podocyte podocyte foot increased width, abnormal	nphs1^{mss103/mss103} (AB)	standard conditions	FIGURE 3 from Lee et al., 2022
mesonephric podocyte mesonephric podocyte development decreased process quality, abnormal	nphs1^{mss103/mss103} (AB)	standard conditions	FIGURE 3 from Lee et al., 2022
heart edematous, abnormal	nphs1^{mss103/mss103} (AB)	standard conditions	FIGURE 1 from Lee et al., 2022
pronephric podocyte podocyte development decreased process quality, abnormal	nphs1^{mss103/mss103} (AB)	standard conditions	FIGURE 3 from Lee et al., 2022
mesonephric podocyte lacks all parts of type mesonephric podocyte slit diaphragm, abnormal	nphs1^{mss103/mss103} (AB)	standard conditions	FIGURE 3 from Lee et al., 2022
orbital region edematous, abnormal	nphs1^{mss103/mss103} (AB)	standard conditions	FIGURE 1 from Lee et al., 2022
pronephric podocyte podocyte foot increased width, abnormal	nphs1^{mss103/mss103} (AB)	standard conditions	FIGURE 3 from Lee et al., 2022
whole organism edematous, abnormal	nphs1^{mss103/mss103} (AB)	standard conditions	FIGURE 1 from Lee et al., 2022
pronephric podocyte lacks all parts of type pronephric podocyte slit diaphragm, abnormal	nphs1^{mss103/mss103} (AB)	standard conditions	FIGURE 3 from Lee et al., 2022
pronephric podocyte Ab4-nphs2 labeling spatial pattern, abnormal	nphs1^{mss102/mss102}; zf238Tg	standard conditions	FIGURE 2 from Lee et al., 2022
pronephric podocyte nphs1 expression absent, abnormal	nphs1^{mss102/mss102}; zf238Tg	standard conditions	FIGURE 2 from Lee et al., 2022
pronephric podocyte Ab4-nphs2 labeling spatial pattern, abnormal	nphs1^{mss103/mss103}; zf238Tg	standard conditions	FIGURE 2 from Lee et al., 2022
pronephric podocyte nphs1 expression absent, abnormal	nphs1^{mss103/mss103}; zf238Tg	standard conditions	FIGURE 2 from Lee et al., 2022
renal protein absorption decreased process quality, abnormal	nphs1^{mss102/mss102}; mi1000Tg	standard conditions	FIGURE 4 from Lee et al., 2022
ocular blood vessel blood plasma EGFP expression decreased amount, abnormal	nphs1^{mss102/mss102}; mi1000Tg	standard conditions	FIGURE 4 from Lee et al., 2022
dorsal aorta blood plasma EGFP expression decreased amount, abnormal	nphs1^{mss102/mss102}; mi1000Tg	standard conditions	FIGURE 4 from Lee et al., 2022
renal protein absorption decreased process quality, abnormal	nphs1^{mss103/mss103}; mi1000Tg	standard conditions	FIGURE 4 from Lee et al., 2022
ocular blood vessel blood plasma EGFP expression decreased amount, abnormal	nphs1^{mss103/mss103}; mi1000Tg	standard conditions	FIGURE 4 from Lee et al., 2022
dorsal aorta blood plasma EGFP expression decreased amount, abnormal	nphs1^{mss103/mss103}; mi1000Tg	standard conditions	FIGURE 4 from Lee et al., 2022

Citations