CRISPR

CRISPR1-amfra

ID

ZDB-CRISPR-230905-8

Name

CRISPR1-amfra

Previous Names

None

Target

amfra (1)

Sequence

5' - TATGTCTGCAAACTAGGCCA - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
re31	amfra

Expression

Gene expression in Wild Types + CRISPR1-amfra

No data available

Phenotype

Phenotype resulting from CRISPR1-amfra

No data available

Phenotype of all Fish created by or utilizing CRISPR1-amfra

Phenotype	Fish	Conditions	Figures
whole organism decreased length, abnormal	amfra^re31/re31	control	Fig. 4 from Deng et al., 2023
motor neuron axon decreased branchiness, abnormal	amfra^re31/re31	chemical treatment by environment: simvastatin	Fig. 4 from Deng et al., 2023
thigmotaxis occurrence, ameliorated	amfra^re31/re31	chemical treatment by environment: atorvastatin	Fig. 4 from Deng et al., 2023
motor neuron axon branchiness, ameliorated	amfra^re31/re31	chemical treatment by environment: atorvastatin	Fig. 4 from Deng et al., 2023
cholesterol biosynthetic process increased process quality, abnormal	amfra^re31/re31	standard conditions	Fig. S9 from Deng et al., 2023
whole organism length, ameliorated	amfra^re31/re31	chemical treatment by environment: simvastatin	Fig. 4 from Deng et al., 2023
brain dhcr24 expression decreased amount, abnormal	amfra^re31/re31	standard conditions	Fig. 4 from Deng et al., 2023
brain lss expression decreased amount, abnormal	amfra^re31/re31	standard conditions	Fig. 4 from Deng et al., 2023
brain rough endoplasmic reticulum dilated, abnormal	amfra^re31/re31	control	Fig. 4 from Deng et al., 2023
cholesterol homeostasis disrupted, abnormal	amfra^re31/re31	standard conditions	Fig. S9 from Deng et al., 2023
brain lipid droplet increased amount, abnormal	amfra^re31/re31	control	Fig. 4 from Deng et al., 2023
swim bladder uninflated, abnormal	amfra^re31/re31	standard conditions	Fig. S8 from Deng et al., 2023
brain perinuclear region of cytoplasm increased size, abnormal	amfra^re31/re31	control	Fig. 4 from Deng et al., 2023
brain lipid homeostasis disrupted, abnormal	amfra^re31/re31	control	Fig. 4 from Deng et al., 2023
motor neuron axon decreased branchiness, abnormal	amfra^re31/re31	control	Fig. 4 from Deng et al., 2023
thigmotaxis occurrence, ameliorated	amfra^re31/re31	chemical treatment by environment: simvastatin	Fig. 4 from Deng et al., 2023
thigmotaxis decreased occurrence, abnormal	amfra^re31/re31	control	Fig. 4 from Deng et al., 2023
whole organism length, ameliorated	amfra^re31/re31	chemical treatment by environment: atorvastatin	Fig. 4 from Deng et al., 2023

Citations