CRISPR

CRISPR1-myrf

ID

ZDB-CRISPR-230728-2

Name

CRISPR1-myrf

Previous Names

None

Target

myrf (1)

Sequence

5' - CATTGACACCAGTATCCTGG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
ue70	myrf

Expression

Gene expression in Wild Types + CRISPR1-myrf

No data available

Phenotype

Phenotype resulting from CRISPR1-myrf

No data available

Phenotype of all Fish created by or utilizing CRISPR1-myrf

Phenotype	Fish	Conditions	Figures
neuron myelin sheath decreased thickness, abnormal	myrf^ue70/ue70 (TL)	standard conditions	Figure 2. from Madden et al., 2021
swimming behavior decreased process quality, abnormal	myrf^ue70/ue70 (TL)	standard conditions	Figure 4. from Madden et al., 2021
Mauthner neuron action potential propagation decreased process quality, abnormal	myrf^ue70/ue70 (TL)	standard conditions	Figure 5. from Madden et al., 2021
Mauthner neuron myelin sheath decreased thickness, abnormal	myrf^ue70/ue70 (TL)	standard conditions	Figure 2. from Madden et al., 2021
spinal cord myelination decreased process quality, abnormal	myrf^ue70/ue70 (TL)	standard conditions	Figure 1. , Figure 2. from Madden et al., 2021
auditory behavior decreased process quality, abnormal	myrf^ue70/ue70 (TL)	standard conditions	Figure 4. from Madden et al., 2021
whole organism female sterile, abnormal	myrf^ue70/ue70 (TL)	standard conditions	text only from Madden et al., 2021
female organism gonad absence of anatomical entity, abnormal	myrf^ue70/ue70 (TL)	standard conditions	text only from Madden et al., 2021
brain mbpa expression decreased amount, abnormal	myrf^ue70/ue70 (TL)	standard conditions	Figure 1. from Madden et al., 2021
spinal cord myelin accumulating cell EGFP expression decreased distribution, abnormal	myrf^ue70/ue70; ue2Tg (TL)	standard conditions	Figure 1. from Madden et al., 2021
spinal cord myelination decreased process quality, abnormal	myrf^ue70/ue70; ue2Tg (TL)	standard conditions	Figure 1. from Madden et al., 2021
spinal cord oligodendrocyte GFP expression decreased amount, abnormal	myrf^ue70/ue70; zf3078Tg (TL)	standard conditions	Figure 3. from Madden et al., 2021
oligodendrocyte myelin sheath decreased amount, abnormal	myrf^ue70/ue70; zf3078Tg (TL)	standard conditions	Figure 3. from Madden et al., 2021
spinal cord oligodendrocyte decreased amount, abnormal	myrf^ue70/ue70; zf3078Tg (TL)	standard conditions	Figure 3. from Madden et al., 2021
oligodendrocyte myelin sheath morphology, abnormal	myrf^ue70/ue70; zf3078Tg (TL)	standard conditions	Figure 3. from Madden et al., 2021
oligodendrocyte myelin sheath retracted, abnormal	myrf^ue70/ue70; zf3078Tg (TL)	standard conditions	Figure 3. from Madden et al., 2021
oligodendrocyte myelin sheath decreased length, abnormal	myrf^ue70/ue70; zf3078Tg (TL)	standard conditions	Figure 3. from Madden et al., 2021

Citations