CRISPR

CRISPR2-tfe3b

ID
ZDB-CRISPR-230227-8
Name
CRISPR2-tfe3b
Previous Names
None
Target
Sequence
5' - GGGTACTTGTCCACCACACT - 3'
ZFIN BLAST NCBI BLAST Ensembl UCSC BLAT
Disclaimer
Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.
Note
The second "G" was added. The PAM site was "CGG" at the 3' end.
Genome Resources
None
Target Location
Constructs
No data available
Genomic Features
Genomic Feature Affected Genomic Regions
st125 tfe3b
st126 tfe3b
Expression
Gene expression in Wild Types + CRISPR2-tfe3b
No data available
Phenotype
Phenotype resulting from CRISPR2-tfe3b
No data available
Phenotype of all Fish created by or utilizing CRISPR2-tfe3b
Phenotype Fish Conditions Figures
midbrain microglial cell amount, ameliorated rragast77/st77; tfe3bst125/+ standard conditions Fig. 4 with image from Iyer et al., 2022
midbrain microglial cell amount, ameliorated rragast77/st77; tfe3bst125/st125 standard conditions Fig. 4 with image from Iyer et al., 2022
midbrain microglial cell amount, ameliorated tfe3bst125/+; tfebst120/+; flcnst127/st127 standard conditions Fig. 5 with image from Iyer et al., 2022
midbrain microglial cell amount, ameliorated tfe3bst125/st125; tfebst120/st120; flcnst127/st127 standard conditions Fig. 5 with image from Iyer et al., 2022
microglial cell lysosomal lumen acidification normal occurrence, ameliorated tfe3bst125/st125; tfebst120/st120; flcnst127/st127 standard conditions Fig. 5 with image from Iyer et al., 2022
microglial cell apoeb expression amount, ameliorated tfe3bst125/st125; tfebst120/st120; flcnst127/st127 standard conditions Fig. 5 with image from Iyer et al., 2022
macrophage EGFP expression spatial pattern, ameliorated tfe3bst125/st125; tfebst120/st120; flcnst127/st127; gl22Tg/gl22Tg standard conditions Fig. 5 with image from Iyer et al., 2022
microglial cell EGFP expression spatial pattern, ameliorated tfe3bst125/st125; tfebst120/st120; flcnst127/st127; gl22Tg/gl22Tg standard conditions Fig. 5 with image from Iyer et al., 2022
midbrain microglial cell normal amount, ameliorated rragast77/st77; tfe3ast124/+; tfe3bst125/+; tfebst120/+ standard conditions Fig. 4 with image from Iyer et al., 2022
Citations
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