CRISPR

CRISPR1-stx4

ID

ZDB-CRISPR-221212-1

Name

CRISPR1-stx4

Previous Names

None

Target

stx4 (1)

Sequence

5' - GCTAGGAGTTGCACTTCCAG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
ci1016	stx4

Expression

Gene expression in Wild Types + CRISPR1-stx4

No data available

Phenotype

Phenotype resulting from CRISPR1-stx4

No data available

Phenotype of all Fish created by or utilizing CRISPR1-stx4

Phenotype	Fish	Conditions	Figures
otic vesicle malformed, abnormal	stx4^{ci1016/ci1016}	standard conditions	Fig. 2 from Perl et al., 2022
heart contraction frequency, ameliorated	stx4^{ci1016/ci1016}	chemical treatment by environment: Bay-K-8644	Fig. 5 , Fig. 6 from Perl et al., 2022
heart contraction decreased frequency, exacerbated	stx4^{ci1016/ci1016}	chemical treatment by environment: nifedipine	Fig. 5 from Perl et al., 2022
cardiac muscle cell vesicle docking decreased process quality, abnormal	stx4^{ci1016/ci1016}	standard conditions	Fig. 3 from Perl et al., 2022
heart looping decreased process quality, abnormal	stx4^{ci1016/ci1016}	standard conditions	Fig. 2 from Perl et al., 2022
heart contraction decreased frequency, exacerbated	stx4^{ci1016/ci1016}	chemical treatment by environment: caffeine, chemical treatment by environment: thapsigargin	Fig. 5 from Perl et al., 2022
cardiac muscle cell plasma membrane stx4 expression spatial pattern, abnormal	stx4^{ci1016/ci1016}	standard conditions	Fig. 3 from Perl et al., 2022
heart contraction decreased frequency, abnormal	stx4^{ci1016/ci1016}	standard conditions	Fig. 4 , Fig. 5 , Fig. 6 from Perl et al., 2022
ventricular myocardium regulation of cardiac muscle contraction by calcium ion signaling decreased process quality, abnormal	stx4^{ci1016/ci1016}	standard conditions	Fig. 4 from Perl et al., 2022
heart contraction decreased frequency, abnormal	stx4^{ci1016/ci1016}	chemical treatment by environment: NNC 55-0396 dihydrochloride	Fig. 5 from Perl et al., 2022
cardiac muscle cell plasma membrane stx4 expression decreased amount, abnormal	stx4^{ci1016/ci1016}	standard conditions	Fig. 3 from Perl et al., 2022
head edematous, abnormal	stx4^{ci1016/ci1016}	standard conditions	Fig. 2 from Perl et al., 2022
atrial myocardium regulation of cardiac muscle contraction by calcium ion signaling decreased process quality, abnormal	stx4^{ci1016/ci1016}	standard conditions	Fig. 4 from Perl et al., 2022
brain lacks all parts of type midbrain hindbrain boundary, abnormal	stx4^{ci1016/ci1016}	standard conditions	Fig. 2 from Perl et al., 2022
heart contraction frequency, ameliorated	stx4^{ci1016/ci1016}; ci1018Tg/+	standard conditions	Fig. 6 from Perl et al., 2022
heart contraction frequency, ameliorated	stx4^{ci1016/ci1016}; ci1019Tg/+	chemical treatment by environment: Bay-K-8644	Fig. 6 from Perl et al., 2022
heart contraction decreased frequency, abnormal	stx4^{ci1016/ci1016}; ci1019Tg/+	standard conditions	Fig. 6 from Perl et al., 2022

Citations