CRISPR

CRISPR1-grb2b

ID

ZDB-CRISPR-220408-4

Name

CRISPR1-grb2b

Previous Names

None

Target

grb2b (1)

Sequence

5' - GATGAGCTGAGTTTTAAACG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
mu404	grb2b

Expression

Gene expression in Wild Types + CRISPR1-grb2b

No data available

Phenotype

Phenotype resulting from CRISPR1-grb2b

No data available

Phenotype of all Fish created by or utilizing CRISPR1-grb2b

Phenotype	Fish	Conditions	Figures
thoracic duct absent, abnormal	grb2b^mu404/mu404	standard conditions	Fig. 3 from Mauri et al., 2021
trunk vascular lymphangioblast absent, abnormal	grb2b^mu404/mu404	standard conditions	Fig. 3 from Mauri et al., 2021
horizontal myoseptum vascular lymphangioblast decreased amount, abnormal	grb2b^hu6987/+; grb2b^mu404/+; hu5333Tg; hu7135Tg	standard conditions	Fig. 1 from Mauri et al., 2021
intersegmental artery decreased amount, abnormal	grb2b^mu404/+; hu5333Tg; hu7135Tg	standard conditions	Fig. 8 from Mauri et al., 2021
intersegmental vein increased amount, abnormal	grb2b^mu404/+; hu5333Tg; hu7135Tg	standard conditions	Fig. 8 from Mauri et al., 2021
vascular lymphangioblast decreased amount, abnormal	grb2b^mu404/+; hu5333Tg; hu7135Tg	standard conditions	Fig. 4 from Mauri et al., 2021
posterior cardinal vein blood vessel endothelial cell migration decreased occurrence, abnormal	grb2b^mu404/mu404; hu5333Tg; hu7135Tg	standard conditions	Fig. 6 from Mauri et al., 2021
intersegmental vein decreased amount, abnormal	grb2b^mu404/mu404; hu5333Tg; hu7135Tg	standard conditions	Fig. 8 from Mauri et al., 2021
posterior cardinal vein sprouting angiogenesis disrupted, abnormal	grb2b^mu404/mu404; hu5333Tg; hu7135Tg	standard conditions	Fig. 6 from Mauri et al., 2021
intersegmental artery increased amount, abnormal	grb2b^mu404/mu404; hu5333Tg; hu7135Tg	standard conditions	Fig. 8 from Mauri et al., 2021
posterior cardinal vein blood vessel endothelial cell ab9-mapk labeling decreased amount, abnormal	grb2b^mu404/mu404; hu7135Tg	standard conditions	Fig. 5 from Mauri et al., 2021
posterior cardinal vein blood vessel endothelial cell ab5-prox1 labeling decreased amount, abnormal	grb2b^mu404/mu404; hu7135Tg	standard conditions	Fig. 5 from Mauri et al., 2021
trunk vascular lymphangioblast absent, abnormal	grb2a^mu405/+; grb2b^mu404/mu404	standard conditions	Fig. 3 from Mauri et al., 2021
thoracic duct absent, abnormal	grb2a^mu405/+; grb2b^mu404/mu404	standard conditions	Fig. 3 from Mauri et al., 2021
trunk vascular lymphangioblast absent, abnormal	grb2a^mu405/mu405; grb2b^mu404/mu404	standard conditions	Fig. 3 from Mauri et al., 2021
horizontal myoseptum vascular lymphangioblast decreased amount, exacerbated	grb2b^mu404/+; hu5333Tg; hu7135Tg + MO3-flt4	standard conditions	Fig. 4 from Mauri et al., 2021

Citations