CRISPR

CRISPR1-bbs2

ID

ZDB-CRISPR-210810-1

Name

CRISPR1-bbs2

Previous Names

None

Target

bbs2 (1)

Sequence

5' - GGAGGAAACTGTGCTCTTCA - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
lri82	bbs2

Expression

Gene expression in Wild Types + CRISPR1-bbs2

No data available

Phenotype

Phenotype resulting from CRISPR1-bbs2

No data available

Phenotype of all Fish created by or utilizing CRISPR1-bbs2

Phenotype	Fish	Conditions	Figures
retina photoreceptor outer segment layer disorganized, abnormal	bbs2^lri82/lri82	standard conditions	Fig. 3, Fig. 4 from Song et al., 2020
retina atf3 expression increased amount, abnormal	bbs2^lri82/lri82	standard conditions	Figure 5 from Grabinski et al., 2023
retina irf1b expression increased amount, abnormal	bbs2^lri82/lri82	standard conditions	Figure 5 from Grabinski et al., 2023
optokinetic behavior decreased process quality, abnormal	bbs2^lri82/lri82	standard conditions	Fig. 2 from Song et al., 2020
retina cd44a expression increased amount, abnormal	bbs2^lri82/lri82	standard conditions	Figure 5 from Grabinski et al., 2023
optic choroid microglial cell increased amount, abnormal	bbs2^lri82/lri82	standard conditions	Fig. 6 from Song et al., 2020
retinal cone cell decreased amount, abnormal	bbs2^lri82/lri82	light damage: eye photoreceptor cell	Fig. 7, Fig. 8 from Song et al., 2020
retina photoreceptor outer segment layer decreased length, abnormal	bbs2^lri82/lri82	standard conditions	Fig. 3 from Song et al., 2020
retinal pigmented epithelium microglial cell increased amount, abnormal	bbs2^lri82/lri82	standard conditions	Fig. 6 from Song et al., 2020
retina cell population proliferation increased process quality, abnormal	bbs2^lri82/lri82	standard conditions	Fig. 4 from Song et al., 2020
retina PCNA complex ab2-pcna labeling increased amount, abnormal	bbs2^lri82/lri82	standard conditions	Fig. 4 from Song et al., 2020
retina atf5a expression increased amount, abnormal	bbs2^lri82/lri82	standard conditions	Figure 5 from Grabinski et al., 2023
whole organism decreased length, abnormal	bbs2^lri82/lri82	standard conditions	Fig. 1 from Song et al., 2020
photoreceptor outer segment layer microglial cell increased amount, abnormal	bbs2^lri82/lri82	standard conditions	Fig. 6 from Song et al., 2020
retina il11a expression increased amount, abnormal	bbs2^lri82/lri82	standard conditions	Figure 5 from Grabinski et al., 2023
retina fosab expression increased amount, abnormal	bbs2^lri82/lri82	standard conditions	Figure 5 from Grabinski et al., 2023
vertebral column curved, abnormal	bbs2^lri82/lri82	standard conditions	Fig. 1 from Song et al., 2020
retina jun expression increased amount, abnormal	bbs2^lri82/lri82	standard conditions	Figure 5 from Grabinski et al., 2023
retina photoreceptor outer segment layer decreased thickness, abnormal	bbs2^lri82/lri82	standard conditions	Fig. 4 from Song et al., 2020
Muller cell cell population proliferation increased process quality, abnormal	bbs2^lri82/lri82	light damage: eye photoreceptor cell	Fig. 7, Fig. 8 from Song et al., 2020
retinal inner plexiform layer Ab1-stx3 labeling decreased amount, abnormal	bbs2^lri82/lri82	standard conditions	Fig. 5 from Song et al., 2020
retina stat1a expression increased amount, abnormal	bbs2^lri82/lri82	standard conditions	Figure 5 from Grabinski et al., 2023
retina mych expression increased amount, abnormal	bbs2^lri82/lri82	standard conditions	Figure 5 from Grabinski et al., 2023
retina retinal cone cell decreased amount, abnormal	bbs2^lri82/lri82	standard conditions	Fig. 4 from Song et al., 2020
retina junbb expression increased amount, abnormal	bbs2^lri82/lri82	standard conditions	Figure 5 from Grabinski et al., 2023
vertebral column kinked, abnormal	bbs2^lri82/lri82	standard conditions	Fig. 1 from Song et al., 2020
retina irf9 expression increased amount, abnormal	bbs2^lri82/lri82	standard conditions	Figure 5 from Grabinski et al., 2023
retina PCNA complex ab2-pcna labeling increased distribution, abnormal	bbs2^lri82/lri82	standard conditions	Fig. 4 from Song et al., 2020
retina myclb expression increased amount, abnormal	bbs2^lri82/lri82	standard conditions	Figure 5 from Grabinski et al., 2023
photoreceptor outer segment layer retinal rod cell Ab1-stx3 labeling increased amount, abnormal	bbs2^lri82/lri82	standard conditions	Fig. 5 from Song et al., 2020

Citations