CRISPR

CRISPR4-nkx3-2

ID

ZDB-CRISPR-210316-7

Name

CRISPR4-nkx3-2

Previous Names

None

Target

nkx3-2 (1)

Sequence

5' - GAGCGCTTCTTCCGCTGCTT - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
ua5011	nkx3-2

Expression

Gene expression in Wild Types + CRISPR4-nkx3-2

No data available

Phenotype

Phenotype resulting from CRISPR4-nkx3-2

No data available

Phenotype of all Fish created by or utilizing CRISPR4-nkx3-2

Phenotype	Fish	Conditions	Figures
caudal vertebra fused with caudal vertebra, abnormal	nkx3-2^{ua5011/ua5011}	standard conditions	Fig. 2 from Smeeton et al., 2021
neural spine decreased length, abnormal	nkx3-2^{ua5011/ua5011}	standard conditions	Fig. 2 from Smeeton et al., 2021
rib alignment rib, abnormal	nkx3-2^{ua5011/ua5011}	standard conditions	Fig. 2 from Smeeton et al., 2021
neural spine ossified, abnormal	nkx3-2^{ua5011/ua5011}	standard conditions	Fig. 2 from Smeeton et al., 2021
caudal vertebra rotated, abnormal	nkx3-2^{ua5011/ua5011}	standard conditions	Fig. 2 from Smeeton et al., 2021
ventral mandibular arch decreased length, abnormal	nkx3-2^{ua5011/ua5011} (AB)	standard conditions	Fig. 2, Fig. 3 from Miyashita et al., 2020
basihyal bone anteroventral orientation, abnormal	nkx3-2^{ua5011/ua5011} (AB)	standard conditions	Fig. 2, Fig. 5 from Miyashita et al., 2020
mouth open, abnormal	nkx3-2^{ua5011/ua5011} (AB)	standard conditions	Fig. 2, Fig. 5 from Miyashita et al., 2020
infraorbital flared, abnormal	nkx3-2^{ua5011/ua5011} (AB)	standard conditions	Fig. 5 from Miyashita et al., 2020
ossification increased occurrence, abnormal	nkx3-2^{ua5011/ua5011} (AB)	standard conditions	Fig. 2, Fig. 5 from Miyashita et al., 2020
quadrate fused with anguloarticular, abnormal	nkx3-2^{ua5011/ua5011} (AB)	standard conditions	Fig. 2, Fig. 3, Fig. 5 from Miyashita et al., 2020
anguloarticular increased size, abnormal	nkx3-2^{ua5011/ua5011} (AB)	standard conditions	Fig. 5 from Miyashita et al., 2020
dentary anteroventral orientation, abnormal	nkx3-2^{ua5011/ua5011} (AB)	standard conditions	Fig. 3 from Miyashita et al., 2020
ventral mandibular arch position, abnormal	nkx3-2^{ua5011/ua5011} (AB)	standard conditions	Fig. 3 from Miyashita et al., 2020
supraorbital bone increased size, abnormal	nkx3-2^{ua5011/ua5011} (AB)	standard conditions	Fig. 5 from Miyashita et al., 2020
premaxilla posteroventral orientation, abnormal	nkx3-2^{ua5011/ua5011} (AB)	standard conditions	Fig. 2 from Miyashita et al., 2020
cranium increased width, abnormal	nkx3-2^{ua5011/ua5011} (AB)	standard conditions	Fig. 2, Fig. 4, Fig. 5 from Miyashita et al., 2020
naris mislocalised posteriorly, abnormal	nkx3-2^{ua5011/ua5011} (AB)	standard conditions	Fig. 2, Fig. 5 from Miyashita et al., 2020
retroarticular process absent, abnormal	nkx3-2^{ua5011/ua5011} (AB)	standard conditions	Fig. 3 from Miyashita et al., 2020
infraorbital increased size, abnormal	nkx3-2^{ua5011/ua5011} (AB)	standard conditions	Fig. 5 from Miyashita et al., 2020
ceratobranchial bone lateral orientation, abnormal	nkx3-2^{ua5011/ua5011} (AB)	standard conditions	Fig. 5 from Miyashita et al., 2020
cranium decreased length, abnormal	nkx3-2^{ua5011/ua5011} (AB)	standard conditions	Fig. 2, Fig. 4, Fig. 5 from Miyashita et al., 2020
adult feeding behavior alternation, abnormal	nkx3-2^{ua5011/ua5011} (AB)	standard conditions	Fig. 6 from Miyashita et al., 2020
supraorbital bone flared, abnormal	nkx3-2^{ua5011/ua5011} (AB)	standard conditions	Fig. 5 from Miyashita et al., 2020
vertebral column malformed, abnormal	nkx3-2^{ua5011/ua5011} (AB)	standard conditions	Fig. S1 from Miyashita et al., 2020
kinethmoid bone absent, abnormal	nkx3-2^{ua5011/ua5011} (AB)	standard conditions	Fig. 2, Fig. 5 from Miyashita et al., 2020
basihyal bone increased size, abnormal	nkx3-2^{ua5011/ua5011} (AB)	standard conditions	Fig. 5 from Miyashita et al., 2020
Meckel's cartilage-palatoquadrate cartilage joint absent, abnormal	nkx3-2^{ua5011/ua5011} (AB)	standard conditions	Fig. 2 from Miyashita et al., 2020
exoccipital increased size, abnormal	nkx3-2^{ua5011/ua5011} (AB)	standard conditions	Fig. 5 from Miyashita et al., 2020
ventral mandibular arch splayed ventral, abnormal	nkx3-2^{ua5011/ua5011} (AB)	standard conditions	Fig. 2, Fig. 3 from Miyashita et al., 2020
maxilla posteroventral orientation, abnormal	nkx3-2^{ua5011/ua5011} (AB)	standard conditions	Fig. 2 from Miyashita et al., 2020
quadrate-anguloarticular joint absent, abnormal	nkx3-2^{ua5011/ua5011} (AB)	standard conditions	Fig. 2, Fig. 5 from Miyashita et al., 2020
palatoquadrate cartilage fused with Meckel's cartilage, abnormal	nkx3-2^{ua5011/ua5011}; ba2Tg; ua3140Tg	standard conditions	Fig. 1 from Miyashita et al., 2020
retroarticular process absent, abnormal	nkx3-2^{ua5011/ua5011}; ba2Tg; ua3140Tg	standard conditions	Fig. 1 from Miyashita et al., 2020
Meckel's cartilage-palatoquadrate cartilage joint absent, abnormal	nkx3-2^{ua5011/ua5011}; ba2Tg; ua3140Tg	standard conditions	Fig. 1 from Miyashita et al., 2020

Citations