CRISPR

CRISPR1-mecom

ID

ZDB-CRISPR-201203-2

Name

CRISPR1-mecom

Previous Names

None

Target

mecom (1)

Sequence

5' - CCCTGGCAACCCTGGCC - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
co1005	mecom
co1013	mecom

Expression

Gene expression in Wild Types + CRISPR1-mecom

No data available

Phenotype

Phenotype resulting from CRISPR1-mecom

No data available

Phenotype of all Fish created by or utilizing CRISPR1-mecom

Phenotype	Fish	Conditions	Figures
pharyngeal arch 3-7 prdm1a expression increased distribution, abnormal	mecom^{co1005/co1005}	standard conditions	Fig. 3 from Shull et al., 2020
pharyngeal arch cartilage col2a1a expression decreased distribution, abnormal	mecom^{co1005/co1005}	standard conditions	Fig. 1 from Shull et al., 2020
chondrocranium chondrocyte disorganized, abnormal	mecom^{co1005/co1005}	standard conditions	Fig. 1 from Shull et al., 2022
opercle decreased size, abnormal	mecom^{co1005/co1005}	standard conditions	Fig. 1 from Shull et al., 2020
chondrocranium cartilage hypoplastic, abnormal	mecom^{co1005/co1005}	standard conditions	Fig. 1 from Shull et al., 2022
cranial cartilage col2a1a expression decreased distribution, abnormal	mecom^{co1005/co1005}	standard conditions	Fig. 1 from Shull et al., 2020
ceratobranchial 5 bone decreased size, abnormal	mecom^{co1005/co1005}	standard conditions	Fig. 1 from Shull et al., 2020
chondrocranium mediolateral intercalation normal process quality, ameliorated	mecom^{co1005/co1005}	chemical treatment by environment: IWR-1-endo	Fig. 6 from Shull et al., 2022
chondrocranium cartilage cartilage morphogenesis decreased process quality, abnormal	mecom^{co1005/co1005}	standard conditions	Fig. 1 from Shull et al., 2022
Meckel's cartilage hypoplastic, abnormal	mecom^{co1005/co1005}	standard conditions	Fig. 1 from Shull et al., 2020
trabecula cranii decreased distance trabecula cranii, abnormal	mecom^{co1005/co1005}	standard conditions	Fig. 1 from Shull et al., 2020
pectoral fin bud prdm1a expression increased distribution, abnormal	mecom^{co1005/co1005}	standard conditions	Fig. 3 from Shull et al., 2020
pharyngeal arch runx2b expression decreased distribution, abnormal	mecom^{co1005/co1005}	standard conditions	Fig. 1 from Shull et al., 2020
Meckel's cartilage decreased distance palatoquadrate cartilage, abnormal	mecom^{co1005/co1005}	standard conditions	Fig. 1 from Shull et al., 2020
opercle bone mineralization decreased occurrence, abnormal	mecom^{co1005/co1005}	standard conditions	Fig. 1 from Shull et al., 2020
cranium runx2b expression decreased distribution, abnormal	mecom^{co1005/co1005}	standard conditions	Fig. 1 from Shull et al., 2020
eye prdm1a expression increased distribution, abnormal	mecom^{co1005/co1005}	standard conditions	Fig. 3 from Shull et al., 2020
head prdm1a expression increased amount, abnormal	mecom^{co1005/co1005}	standard conditions	Fig. 3 from Shull et al., 2020
ceratobranchial 5 bone bone mineralization decreased occurrence, abnormal	mecom^{co1005/co1005}	standard conditions	Fig. 1 from Shull et al., 2020
head prdm16 expression increased amount, abnormal	mecom^{co1005/co1005}	standard conditions	Fig. 3 from Shull et al., 2020
hatching gland prdm1a expression increased distribution, abnormal	mecom^{co1005/co1005}	standard conditions	Fig. 3 from Shull et al., 2020
chondrocranium cartilage hypoplastic, abnormal	mecom^{co1005/co1005}	chemical treatment by environment: IWR-1-endo	Fig. 6 from Shull et al., 2022
chondrocranium mediolateral intercalation decreased process quality, abnormal	mecom^{co1005/co1005}	standard conditions	Fig. 1 , Fig. 6 from Shull et al., 2022
ceratobranchial cartilage hypoplastic, abnormal	mecom^{co1005/co1005}	standard conditions	Fig. 1 from Shull et al., 2020
palatoquadrate cartilage hypoplastic, abnormal	mecom^{co1005/co1005}	standard conditions	Fig. 1 from Shull et al., 2020
Wnt signalosome assembly increased rate, abnormal	mecom^{co1005/co1005}; ba2Tg/ba2Tg	standard conditions	Fig. 3 from Shull et al., 2022
cranial neural crest cell fosab expression increased amount, abnormal	mecom^{co1005/co1005}; ba2Tg/ba2Tg	standard conditions	Fig. 3 from Shull et al., 2022
cranial neural crest apc expression increased amount, abnormal	mecom^{co1005/co1005}; ba2Tg/ba2Tg	standard conditions	Fig. 3 from Shull et al., 2022
cranial neural crest cell jun expression increased amount, abnormal	mecom^{co1005/co1005}; ba2Tg/ba2Tg	standard conditions	Fig. 3 from Shull et al., 2022
cranial neural crest cell dvl3a expression increased amount, abnormal	mecom^{co1005/co1005}; ba2Tg/ba2Tg	standard conditions	Fig. 3 from Shull et al., 2022
cranial neural crest chromatin remodeling decreased process quality, abnormal	mecom^{co1005/co1005}; ba2Tg/ba2Tg	standard conditions	Fig. 5 from Shull et al., 2022
cranial neural crest cell tcf7 expression increased amount, abnormal	mecom^{co1005/co1005}; ba2Tg/ba2Tg	standard conditions	Fig. 3 from Shull et al., 2022
cranial neural crest cell fzd3b expression increased amount, abnormal	mecom^{co1005/co1005}; ba2Tg/ba2Tg	standard conditions	Fig. 3 from Shull et al., 2022
cranial neural crest cell bcl9 expression increased amount, abnormal	mecom^{co1005/co1005}; ba2Tg/ba2Tg	standard conditions	Fig. 3 from Shull et al., 2022
chondrocyte nucleus Ab5-ctnnb1 labeling increased amount, abnormal	mecom^{co1005/co1005}; ba2Tg/ba2Tg	standard conditions	Fig. 4 from Shull et al., 2022
cranial neural crest cell pygo1 expression increased amount, abnormal	mecom^{co1005/co1005}; ba2Tg/ba2Tg	standard conditions	Fig. 3 from Shull et al., 2022
cranial neural crest cell smarca4a expression increased amount, abnormal	mecom^{co1005/co1005}; ba2Tg/ba2Tg	standard conditions	Fig. 3 from Shull et al., 2022
cranial neural crest cell ctnnb1 expression increased amount, abnormal	mecom^{co1005/co1005}; ba2Tg/ba2Tg	standard conditions	Fig. 3 from Shull et al., 2022
cranial neural crest cell pygo2 expression increased amount, abnormal	mecom^{co1005/co1005}; ba2Tg/ba2Tg	standard conditions	Fig. 3 from Shull et al., 2022
cranial neural crest cell lef1 expression increased amount, abnormal	mecom^{co1005/co1005}; ba2Tg/ba2Tg	standard conditions	Fig. 3 from Shull et al., 2022
pharyngeal arch Wnt signalosome mCherry expression increased amount, abnormal	mecom^{co1005/co1005}; ia5Tg/ia5Tg; y1Tg/y1Tg	standard conditions	Fig. 4 from Shull et al., 2022
chondrocranium mediolateral intercalation normal process quality, ameliorated	prdm16^{co1006/co1006}; mecom^{co1005/co1005}	standard conditions	Fig. 6 from Shull et al., 2022
chondrocranium cartilage normal amount, ameliorated	prdm16^{co1006/co1006}; mecom^{co1005/co1005}	standard conditions	Fig. 6 from Shull et al., 2022
palatoquadrate cartilage hypoplastic, abnormal	prdm16^co1006/+; prdm1a^m805/+; mecom^{co1005/co1005}	standard conditions	Fig. 3 from Shull et al., 2020
Meckel's cartilage hypoplastic, abnormal	prdm16^co1006/+; prdm1a^m805/+; mecom^{co1005/co1005}	standard conditions	Fig. 3 from Shull et al., 2020
Meckel's cartilage decreased distance palatoquadrate cartilage, abnormal	prdm16^co1006/+; prdm1a^m805/+; mecom^{co1005/co1005}	standard conditions	Fig. 3 from Shull et al., 2020
ceratobranchial cartilage aplastic, abnormal	prdm16^co1006/+; prdm1a^m805/m805; mecom^co1005/+	standard conditions	Fig. 3 from Shull et al., 2020
palatoquadrate cartilage hypoplastic, abnormal	prdm16^co1006/+; prdm1a^m805/m805; mecom^{co1005/co1005}	standard conditions	Fig. 3 from Shull et al., 2020
ethmoid cartilage split, abnormal	prdm16^co1006/+; prdm1a^m805/m805; mecom^{co1005/co1005}	standard conditions	Fig. 3 from Shull et al., 2020
Meckel's cartilage hypoplastic, abnormal	prdm16^co1006/+; prdm1a^m805/m805; mecom^{co1005/co1005}	standard conditions	Fig. 3 from Shull et al., 2020
ceratobranchial cartilage aplastic, abnormal	prdm16^co1006/+; prdm1a^m805/m805; mecom^{co1005/co1005}	standard conditions	Fig. 3 from Shull et al., 2020
Meckel's cartilage decreased distance palatoquadrate cartilage, abnormal	prdm16^{co1006/co1006}; prdm1a^m805/+; mecom^co1005/+	standard conditions	Fig. 3 from Shull et al., 2020
Meckel's cartilage aplastic/hypoplastic, abnormal	prdm16^{co1006/co1006}; prdm1a^m805/+; mecom^co1005/+	standard conditions	Fig. 3 from Shull et al., 2020
Meckel's cartilage decreased distance palatoquadrate cartilage, abnormal	prdm16^{co1006/co1006}; prdm1a^m805/+; mecom^{co1005/co1005}	standard conditions	Fig. 3 from Shull et al., 2020
Meckel's cartilage aplastic/hypoplastic, abnormal	prdm16^{co1006/co1006}; prdm1a^m805/+; mecom^{co1005/co1005}	standard conditions	Fig. 3 from Shull et al., 2020
ethmoid cartilage split, abnormal	prdm16^{co1006/co1006}; prdm1a^m805/m805; mecom^co1005/+	standard conditions	Fig. 3 from Shull et al., 2020
Meckel's cartilage decreased distance palatoquadrate cartilage, abnormal	prdm16^{co1006/co1006}; prdm1a^m805/m805; mecom^co1005/+	standard conditions	Fig. 3 from Shull et al., 2020
ceratobranchial cartilage aplastic, abnormal	prdm16^{co1006/co1006}; prdm1a^m805/m805; mecom^co1005/+	standard conditions	Fig. 3 from Shull et al., 2020
Meckel's cartilage aplastic/hypoplastic, abnormal	prdm16^{co1006/co1006}; prdm1a^m805/m805; mecom^co1005/+	standard conditions	Fig. 3 from Shull et al., 2020
palatoquadrate cartilage hypoplastic, abnormal	prdm16^{co1006/co1006}; prdm1a^m805/m805; mecom^{co1005/co1005}	standard conditions	Fig. 3 from Shull et al., 2020
ethmoid cartilage split, abnormal	prdm16^{co1006/co1006}; prdm1a^m805/m805; mecom^{co1005/co1005}	standard conditions	Fig. 3 from Shull et al., 2020
Meckel's cartilage hypoplastic, abnormal	prdm16^{co1006/co1006}; prdm1a^m805/m805; mecom^{co1005/co1005}	standard conditions	Fig. 3 from Shull et al., 2020
Meckel's cartilage decreased distance palatoquadrate cartilage, abnormal	prdm16^{co1006/co1006}; prdm1a^m805/m805; mecom^{co1005/co1005}	standard conditions	Fig. 3 from Shull et al., 2020
ceratobranchial cartilage aplastic, abnormal	prdm16^{co1006/co1006}; prdm1a^m805/m805; mecom^{co1005/co1005}	standard conditions	Fig. 3 from Shull et al., 2020
Meckel's cartilage aplastic/hypoplastic, abnormal	prdm16^{co1006/co1006}; prdm1a^m805/m805; mecom^{co1005/co1005}	standard conditions	Fig. 3 from Shull et al., 2020

Citations