CRISPR

CRISPR1-padi2

ID

ZDB-CRISPR-201020-5

Name

CRISPR1-padi2

Previous Names

None

Target

padi2 (1)

Sequence

5' - GGGAACAGACACGCTGACGC - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "AGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
uwm44	padi2

Expression

Gene expression in Wild Types + CRISPR1-padi2

No data available

Phenotype

Phenotype resulting from CRISPR1-padi2

No data available

Phenotype of all Fish created by or utilizing CRISPR1-padi2

Phenotype	Fish	Conditions	Figures
regenerating fin padi2 expression absent, abnormal	padi2^uwm44/uwm44	surgical manipulation: caudal fin	Fig. S3 from Golenberg et al., 2020
caudal fin increased length, abnormal	padi2^uwm44/uwm44	standard conditions	Fig. 2 from Golenberg et al., 2020
regenerating fin histone arginine deiminase activity arrested, abnormal	padi2^uwm44/uwm44	surgical manipulation: caudal fin	Fig. 3 from Golenberg et al., 2020
myotome neuromuscular junction increased amount, abnormal	padi2^uwm44/uwm44	standard conditions	Fig. S2 from Golenberg et al., 2020
blastema Ab8-h4 labeling absent, abnormal	padi2^uwm44/uwm44	surgical manipulation: caudal fin	Fig. 3 from Golenberg et al., 2020
wound healing process quality, abnormal	padi2^uwm44/uwm44	surgical manipulation: caudal fin	Fig. 4 from Golenberg et al., 2020
whole organism padi2 expression decreased amount, abnormal	padi2^uwm44/uwm44	standard conditions	Fig. 1 from Golenberg et al., 2020
regenerating fin cell population proliferation decreased occurrence, abnormal	padi2^uwm44/uwm44	surgical manipulation: caudal fin	Fig. 4 from Golenberg et al., 2020
whole organism padi2 expression absent, abnormal	padi2^uwm44/uwm44	standard conditions	Fig. 1 from Golenberg et al., 2020
fin regeneration decreased process quality, abnormal	padi2^uwm44/uwm44	surgical manipulation: caudal fin	Fig. 2, Fig. 4 from Golenberg et al., 2020
regenerating fin decreased length, abnormal	padi2^uwm44/uwm44	surgical manipulation: caudal fin	Fig. 2 from Golenberg et al., 2020
regenerating fin neutrophil increased amount, abnormal	padi2^uwm44/uwm44; uwm40Tg	surgical manipulation: caudal fin	Fig. 2 from Golenberg et al., 2020
blastema macrophage aggregated, abnormal	padi2^uwm44/uwm44; uwm43Tg	surgical manipulation: caudal fin	Fig. 2 from Golenberg et al., 2020
regenerating fin macrophage increased amount, abnormal	padi2^uwm44/uwm44; uwm43Tg	surgical manipulation: caudal fin	Fig. 2 from Golenberg et al., 2020

Citations