CRISPR

CRISPR1-cacna2d4b

ID

ZDB-CRISPR-200604-3

Name

CRISPR1-cacna2d4b

Previous Names

None

Target

cacna2d4b (1)

Sequence

5' - GGATGTGAGCGGCAGCATGA - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
zh6	cacna2d4b

Expression

Gene expression in Wild Types + CRISPR1-cacna2d4b

No data available

Phenotype

Phenotype resulting from CRISPR1-cacna2d4b

No data available

Phenotype of all Fish created by or utilizing CRISPR1-cacna2d4b

Phenotype	Fish	Conditions	Figures
retinal outer plexiform layer cacna1fa expression decreased amount, abnormal	cacna2d4b^zh6/zh6 (TU)	standard conditions	Fig. 2 from Schlegel et al., 2019
retinal photoreceptor layer ctbp2l expression mislocalised, abnormal	cacna2d4b^zh6/zh6 (TU)	standard conditions	Fig. 6 from Schlegel et al., 2019
eye decreased size, abnormal	cacna2d4b^zh6/zh6 (TU)	standard conditions	Fig. 5 from Schlegel et al., 2019
retinal photoreceptor layer cacna1fa expression mislocalised, abnormal	cacna2d4b^zh6/zh6 (TU)	standard conditions	Fig. 2 from Schlegel et al., 2019
retinal outer plexiform layer cacna1fa expression decreased amount, abnormal	cacna2d4a^zh5/zh5; cacna2d4b^zh6/zh6 (TU)	standard conditions	Fig. 2 from Schlegel et al., 2019
retinal cone cell development decreased process quality, abnormal	cacna2d4a^zh5/zh5; cacna2d4b^zh6/zh6 (TU)	standard conditions	Fig. 3 , Fig. 4 , Fig. 7 from Schlegel et al., 2019
eye decreased size, abnormal	cacna2d4a^zh5/zh5; cacna2d4b^zh6/zh6 (TU)	standard conditions	Fig. 5 from Schlegel et al., 2019
retinal photoreceptor layer cacna1fa expression mislocalised, abnormal	cacna2d4a^zh5/zh5; cacna2d4b^zh6/zh6 (TU)	standard conditions	Fig. 2 from Schlegel et al., 2019
retinal outer plexiform layer has fewer parts of type eye photoreceptor cell synaptic ribbon, abnormal	cacna2d4a^zh5/zh5; cacna2d4b^zh6/zh6 (TU)	standard conditions	Fig. 7 from Schlegel et al., 2019
retinal photoreceptor layer ctbp2l expression mislocalised, abnormal	cacna2d4a^zh5/zh5; cacna2d4b^zh6/zh6 (TU)	standard conditions	Fig. 6 from Schlegel et al., 2019
eye photoreceptor cell synaptic ribbon separated from eye photoreceptor cell photoreceptor ribbon synapse, abnormal	cacna2d4a^zh5/zh5; cacna2d4b^zh6/zh6 (TU)	standard conditions	Fig. 7 from Schlegel et al., 2019
retinal cone cell chemical synaptic transmission decreased process quality, abnormal	cacna2d4a^zh5/zh5; cacna2d4b^zh6/zh6 (TU)	standard conditions	Fig. 3 , Fig. 4 from Schlegel et al., 2019

Citations