CRISPR

CRISPR1-cacna2d4a

ID
ZDB-CRISPR-200604-2
Name
CRISPR1-cacna2d4a
Previous Names
None
Target
Sequence
5' - GGAACCCATCGTCAAGATGG - 3'
ZFIN BLAST NCBI BLAST Ensembl UCSC BLAT
Disclaimer
Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.
Note
None
Genome Resources
None
Target Location
Constructs
No data available
Genomic Features
Genomic Feature Affected Genomic Regions
zh5 cacna2d4a
Expression
Gene expression in Wild Types + CRISPR1-cacna2d4a
No data available
Phenotype
Phenotype resulting from CRISPR1-cacna2d4a
No data available
Phenotype of all Fish created by or utilizing CRISPR1-cacna2d4a
Phenotype Fish Conditions Figures
eye decreased size, abnormal cacna2d4azh5/zh5 (TU) standard conditions Fig. 5 with image from Schlegel et al., 2019
retinal outer plexiform layer cacna1fa expression decreased amount, abnormal cacna2d4azh5/zh5; cacna2d4bzh6/zh6 (TU) standard conditions Fig. 2 with image from Schlegel et al., 2019
retinal cone cell development decreased process quality, abnormal cacna2d4azh5/zh5; cacna2d4bzh6/zh6 (TU) standard conditions Fig. 3 with image,  Fig. 4 with image,  Fig. 7 with image from Schlegel et al., 2019
eye decreased size, abnormal cacna2d4azh5/zh5; cacna2d4bzh6/zh6 (TU) standard conditions Fig. 5 with image from Schlegel et al., 2019
retinal photoreceptor layer cacna1fa expression mislocalised, abnormal cacna2d4azh5/zh5; cacna2d4bzh6/zh6 (TU) standard conditions Fig. 2 with image from Schlegel et al., 2019
retinal outer plexiform layer has fewer parts of type eye photoreceptor cell synaptic ribbon, abnormal cacna2d4azh5/zh5; cacna2d4bzh6/zh6 (TU) standard conditions Fig. 7 with image from Schlegel et al., 2019
retinal photoreceptor layer ctbp2l expression mislocalised, abnormal cacna2d4azh5/zh5; cacna2d4bzh6/zh6 (TU) standard conditions Fig. 6 with image from Schlegel et al., 2019
eye photoreceptor cell synaptic ribbon separated from eye photoreceptor cell photoreceptor ribbon synapse, abnormal cacna2d4azh5/zh5; cacna2d4bzh6/zh6 (TU) standard conditions Fig. 7 with image from Schlegel et al., 2019
retinal cone cell chemical synaptic transmission decreased process quality, abnormal cacna2d4azh5/zh5; cacna2d4bzh6/zh6 (TU) standard conditions Fig. 3 with image,  Fig. 4 with image from Schlegel et al., 2019
Citations
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