CRISPR

CRISPR2-lnc.cnib1

ID

ZDB-CRISPR-200408-3

Name

CRISPR2-lnc.cnib1

Previous Names

None

Target

lnc.cnib1 (1)

Sequence

5' - CTGCTAGCGAGGACACACTG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

WARNING: Wang et al. (2019) produced functional evidence that this lncRNA exists and may be conserved The CRISPR and primers sequences to BLAST to the indicated intronic region in pbx3b, however, there is no evidence that any lncRNAs are in this genomic region.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
zf3098	lnc.cnib1
zf3099	lnc.cnib1

Expression

Gene expression in Wild Types + CRISPR2-lnc.cnib1

No data available

Phenotype

Phenotype resulting from CRISPR2-lnc.cnib1

No data available

Phenotype of all Fish created by or utilizing CRISPR2-lnc.cnib1

Phenotype	Fish	Conditions	Figures
whole organism dead, abnormal	lnc.cnib1^{zf3098/zf3098}	standard conditions	Fig. 3 from Wang et al., 2019
whole organism decreased life span, abnormal	lnc.cnib1^{zf3098/zf3098}	standard conditions	Fig. 3 from Wang et al., 2019
optokinetic behavior decreased occurrence, abnormal	lnc.cnib1^zf3098/+	standard conditions	Fig. 5 from Wang et al., 2019
eye decreased size, abnormal	lnc.cnib1^zf3098/+	standard conditions	Fig. 5 from Wang et al., 2019
optokinetic behavior decreased process quality, abnormal	lnc.cnib1^zf3098/+	standard conditions	Fig. 5 from Wang et al., 2019
hatching decreased occurrence, abnormal	lnc.cnib1^zf3098/+	standard conditions	Fig. 4 from Wang et al., 2019
swimming decreased occurrence, abnormal	lnc.cnib1^zf3098/+	standard conditions	Fig. 4 from Wang et al., 2019
whole organism decreased length, abnormal	lnc.cnib1^zf3098/+	standard conditions	Fig. 4 , Fig. S7 from Wang et al., 2019
swimming decreased linear velocity, abnormal	lnc.cnib1^zf3098/+	standard conditions	Fig. 4 from Wang et al., 2019
optokinetic behavior decreased occurrence, abnormal	lnc.cnib1^zf3099/+	standard conditions	Fig. 5 from Wang et al., 2019
eye decreased size, abnormal	lnc.cnib1^zf3099/+	standard conditions	Fig. 5 from Wang et al., 2019
retina dopaminergic neuron decreased amount, abnormal	lnc.cnib1^zf3099/+	standard conditions	Fig. 5 from Wang et al., 2019
whole organism decreased life span, abnormal	lnc.cnib1^zf3099/+	standard conditions	Fig. 3 from Wang et al., 2019
whole organism eya2 expression decreased amount, abnormal	lnc.cnib1^zf3099/+	standard conditions	Fig. S13 from Wang et al., 2019
swimming decreased linear velocity, abnormal	lnc.cnib1^zf3099/+	standard conditions	Fig. 4 from Wang et al., 2019
hatching decreased occurrence, abnormal	lnc.cnib1^zf3099/+	standard conditions	Fig. 4 from Wang et al., 2019
whole organism foxc1a expression decreased amount, abnormal	lnc.cnib1^zf3099/+	standard conditions	Fig. S13 from Wang et al., 2019
optokinetic behavior decreased process quality, abnormal	lnc.cnib1^zf3099/+	standard conditions	Fig. 5 from Wang et al., 2019
eye lmx1bb expression decreased amount, abnormal	lnc.cnib1^zf3099/+	standard conditions	Fig. 6 from Wang et al., 2019
whole organism decreased length, abnormal	lnc.cnib1^zf3099/+	standard conditions	Fig. 4 , Fig. S7 from Wang et al., 2019
swimming decreased occurrence, abnormal	lnc.cnib1^zf3099/+	standard conditions	Fig. 4 from Wang et al., 2019
brain lmx1bb expression decreased amount, abnormal	lnc.cnib1^zf3099/+	standard conditions	Fig. 6 from Wang et al., 2019
whole organism dead, abnormal	lnc.cnib1^zf3099/+	standard conditions	Fig. 3 from Wang et al., 2019
whole organism pax8 expression decreased amount, abnormal	lnc.cnib1^zf3099/+	standard conditions	Fig. S13 from Wang et al., 2019

Citations