CRISPR

CRISPR1-saa

ID

ZDB-CRISPR-200303-1

Name

CRISPR1-saa

Previous Names

None

Target

saa (1)

Sequence

5' - AAGCGATACCACTGCGCC - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

This CRISPR also appears to target this region in Ensembl 13:19881605-19886206.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
rdu60	saa
rdu61	saa
rdu62	saa

Expression

Gene expression in Wild Types + CRISPR1-saa

No data available

Phenotype

Phenotype resulting from CRISPR1-saa

No data available

Phenotype of all Fish created by or utilizing CRISPR1-saa

Phenotype	Fish	Conditions	Figures
whole organism lcp1 expression increased amount, abnormal	saa^rdu60/rdu60	control	Fig 2 from Murdoch et al., 2019
immature neutrophil increased amount, abnormal	saa^rdu60/rdu60	control	Fig 2 from Murdoch et al., 2019
mature neutrophil decreased amount, abnormal	saa^rdu60/rdu60	control	Fig 2 from Murdoch et al., 2019
gut saa expression decreased amount, abnormal	saa^rdu60/rdu60	control	Fig 4 from Murdoch et al., 2019
whole organism csf3a expression increased amount, abnormal	saa^rdu60/rdu60	control	Fig 2 from Murdoch et al., 2019
whole organism lyz expression increased amount, abnormal	saa^rdu60/rdu60	control	Fig 2 from Murdoch et al., 2019
intestine neutrophil decreased amount, abnormal	saa^rdu60/rdu60; nz50Tg	control	Fig 4 from Murdoch et al., 2019
neutrophil chemotaxis decreased occurrence, abnormal	saa^rdu60/rdu60; nz50Tg	amputation: caudal fin	Fig 4 from Murdoch et al., 2019
neutrophil tnfaip3 expression increased amount, abnormal	saa^rdu60/rdu60; nz117Tg	control	Fig 3 from Murdoch et al., 2019
trunk neutrophil decreased speed, abnormal	saa^rdu60/rdu60; nz117Tg	amputation: caudal fin	Fig 1 from Murdoch et al., 2019
neutrophil pglyrp2 expression decreased amount, abnormal	saa^rdu60/rdu60; nz117Tg	germ free	Fig. 5 from Murdoch et al., 2019
neutrophil tnfa expression decreased amount, abnormal	saa^rdu60/rdu60; nz117Tg	germ free	Fig. 5 from Murdoch et al., 2019
neutrophil increased amount, abnormal	saa^rdu60/rdu60; nz117Tg	control	Fig 2 from Murdoch et al., 2019
neutrophil ncf2 expression decreased amount, abnormal	saa^rdu60/rdu60; nz117Tg	germ free	Fig. 5 from Murdoch et al., 2019
neutrophil pglyrp2 expression increased amount, abnormal	saa^rdu60/rdu60; nz117Tg	control	Fig 3 , Fig. 5 from Murdoch et al., 2019
neutrophil il1b expression increased amount, abnormal	saa^rdu60/rdu60; nz117Tg	control	Fig 3 from Murdoch et al., 2019
neutrophil il10 expression increased amount, abnormal	saa^rdu60/rdu60; nz117Tg	control	Fig 3 from Murdoch et al., 2019
neutrophil ncf2 expression increased amount, abnormal	saa^rdu60/rdu60; nz117Tg	control	Fig 3 , Fig. 5 from Murdoch et al., 2019
neutrophil decreased speed, abnormal	saa^rdu60/rdu60; nz117Tg	amputation: caudal fin	Fig 1 from Murdoch et al., 2019
intestine neutrophil decreased amount, abnormal	saa^rdu60/rdu60; nz117Tg	amputation: caudal fin	Fig 1 from Murdoch et al., 2019
neutrophil tnfa expression increased amount, abnormal	saa^rdu60/rdu60; nz117Tg	control	Fig 3 , Fig. 5 from Murdoch et al., 2019
neutrophil mpx expression increased amount, abnormal	saa^rdu60/rdu60; nz117Tg	control	Fig 3 from Murdoch et al., 2019
neutrophil chemotaxis decreased occurrence, abnormal	saa^rdu60/rdu60; nz117Tg	amputation: caudal fin	Fig 1 from Murdoch et al., 2019
neutrophil pglyrp5 expression decreased amount, abnormal	saa^rdu60/rdu60; nz117Tg	germ free	Fig. 5 from Murdoch et al., 2019
neutrophil pglyrp5 expression increased amount, abnormal	saa^rdu60/rdu60; nz117Tg	control	Fig 3 , Fig. 5 from Murdoch et al., 2019
neutrophil amount, ameliorated	saa^rdu60/rdu60; nz50Tg; rdu64Tg	control	Fig 4 from Murdoch et al., 2019
neutrophil chemotaxis occurrence, ameliorated	saa^rdu60/rdu60; nz50Tg; rdu64Tg	amputation: caudal fin	Fig 4 from Murdoch et al., 2019
neutrophil chemotaxis occurrence, ameliorated	saa^rdu60/rdu60; nz50Tg; rdu66Tg	amputation: caudal fin	Fig 4 from Murdoch et al., 2019
intestine neutrophil decreased amount, abnormal	saa^rdu60/rdu60; nz50Tg; rdu66Tg	control	Fig 4 from Murdoch et al., 2019
neutrophil amount, ameliorated	saa^rdu60/rdu60; nz50Tg; rdu67Tg	control	Fig 4 from Murdoch et al., 2019
neutrophil chemotaxis occurrence, ameliorated	saa^rdu60/rdu60; nz50Tg; rdu67Tg	amputation: caudal fin	Fig 4 from Murdoch et al., 2019
neutrophil amount, ameliorated	saa^rdu60/rdu60; nz50Tg; rdu68Tg	control	Fig 4 from Murdoch et al., 2019
neutrophil chemotaxis occurrence, ameliorated	saa^rdu60/rdu60; nz50Tg; rdu68Tg	amputation: caudal fin	Fig 4 from Murdoch et al., 2019
gut saa expression amount, ameliorated	saa^rdu60/rdu60; rdu64Tg	control	Fig 4 from Murdoch et al., 2019
whole organism saa expression increased amount, abnormal	saa^rdu60/rdu60; rdu66Tg	control	Fig 4 from Murdoch et al., 2019
gut saa expression amount, ameliorated	saa^rdu60/rdu60; rdu67Tg	control	Fig 4 from Murdoch et al., 2019
gut saa expression amount, ameliorated	saa^rdu60/rdu60; rdu68Tg	control	Fig 4 from Murdoch et al., 2019

Citations