CRISPR

CRISPR1-gtpbp3

ID
ZDB-CRISPR-191106-1
Name
CRISPR1-gtpbp3
Previous Names
None
Target
Sequence
5' - GGTGGTCCGGGTCAGCGGTC - 3'
Disclaimer
Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.
Note
The PAM sequence is CGG at the 3' end.
Genome Resources
None
Target Location
Constructs
No data available
Genomic Features
Genomic Feature Affected Genomic Regions
zf2239 gtpbp3
Expression
Gene expression in Wild Types + CRISPR1-gtpbp3
No data available
Phenotype
Phenotype resulting from CRISPR1-gtpbp3
No data available
Phenotype of all Fish created by or utilizing CRISPR1-gtpbp3
Phenotype Fish Conditions Figures
whole organism mitochondrion mto1 expression decreased amount, abnormal gtpbp3zf2239/zf2239 (AB) standard conditions Fig. 5 from Chen et al., 2019
whole organism respiratory chain complex II (succinate dehydrogenase) decreased amount, abnormal gtpbp3zf2239/zf2239 (AB) standard conditions Fig. 6 from Chen et al., 2019
whole organism respiratory chain complex I decreased efficiency, abnormal gtpbp3zf2239/zf2239 (AB) standard conditions Fig. 7 from Chen et al., 2019
whole organism mitochondrion mt-co2 expression decreased amount, abnormal gtpbp3zf2239/zf2239 (AB) standard conditions Fig. 5 from Chen et al., 2019
whole organism mitochondrion trmu expression decreased amount, abnormal gtpbp3zf2239/zf2239 (AB) standard conditions Fig. 5 from Chen et al., 2019
whole organism mitochondrion mt-nd5 expression decreased amount, abnormal gtpbp3zf2239/zf2239 (AB) standard conditions Fig. 5 from Chen et al., 2019
heart nppb expression increased amount, abnormal gtpbp3zf2239/zf2239 (AB) standard conditions Fig. 9 from Chen et al., 2019
mitochondrial electron transport, cytochrome c to oxygen decreased occurrence, abnormal gtpbp3zf2239/zf2239 (AB) standard conditions Fig. 10 from Chen et al., 2019
whole organism respiratory chain complex IV decreased efficiency, abnormal gtpbp3zf2239/zf2239 (AB) standard conditions Fig. 7 from Chen et al., 2019
whole organism proton-transporting ATP synthase complex decreased efficiency, abnormal gtpbp3zf2239/zf2239 (AB) standard conditions Fig. 7 from Chen et al., 2019
whole organism mitochondrion yars2 expression decreased amount, abnormal gtpbp3zf2239/zf2239 (AB) standard conditions Fig. 5 from Chen et al., 2019
whole organism obsolete mitochondrial respiratory chain complex II, succinate dehydrogenase complex (ubiquinone) decreased efficiency, abnormal gtpbp3zf2239/zf2239 (AB) standard conditions Fig. 7 from Chen et al., 2019
cardiac muscle disorganized, abnormal gtpbp3zf2239/zf2239 (AB) standard conditions Fig. 9 from Chen et al., 2019
whole organism respiratory chain complex IV decreased amount, abnormal gtpbp3zf2239/zf2239 (AB) standard conditions Fig. 6 from Chen et al., 2019
whole organism mitochondrion kars1 expression decreased amount, abnormal gtpbp3zf2239/zf2239 (AB) standard conditions Fig. 5 from Chen et al., 2019
whole organism mitochondrion tufm expression decreased amount, abnormal gtpbp3zf2239/zf2239 (AB) standard conditions Fig. 5 from Chen et al., 2019
cardiac muscle hypertrophic, abnormal gtpbp3zf2239/zf2239 (AB) standard conditions Fig. 9 from Chen et al., 2019
mitochondrial lysyl-tRNA aminoacylation increased occurrence, abnormal gtpbp3zf2239/zf2239 (AB) standard conditions Fig. 4 from Chen et al., 2019
mitochondrial methionyl-tRNA aminoacylation increased occurrence, abnormal gtpbp3zf2239/zf2239 (AB) standard conditions Fig. 4 from Chen et al., 2019
mitochondrial tryptophanyl-tRNA aminoacylation increased occurrence, abnormal gtpbp3zf2239/zf2239 (AB) standard conditions Fig. 4 from Chen et al., 2019
whole organism clpp expression increased amount, abnormal gtpbp3zf2239/zf2239 (AB) standard conditions Fig. 6 from Chen et al., 2019
mitochondrial electron transport, succinate to ubiquinone decreased occurrence, abnormal gtpbp3zf2239/zf2239 (AB) standard conditions Fig. 10 from Chen et al., 2019
whole organism afg3l2 expression increased amount, abnormal gtpbp3zf2239/zf2239 (AB) standard conditions Fig. 6 from Chen et al., 2019
whole organism mitochondrion atp5f1c expression decreased amount, abnormal gtpbp3zf2239/zf2239 (AB) standard conditions Fig. 5 from Chen et al., 2019
whole organism mitochondrion mt-nd6 expression decreased amount, abnormal gtpbp3zf2239/zf2239 (AB) standard conditions Fig. 5 from Chen et al., 2019
cardiac muscle cell increased size, abnormal gtpbp3zf2239/zf2239 (AB) standard conditions Fig. 9 from Chen et al., 2019
heart mitochondrial crista shape, abnormal gtpbp3zf2239/zf2239 (AB) standard conditions Fig. 10 from Chen et al., 2019
leucyl-tRNA aminoacylation increased occurrence, abnormal gtpbp3zf2239/zf2239 (AB) standard conditions Fig. 4 from Chen et al., 2019
whole organism respiratory chain complex II (succinate dehydrogenase) decreased efficiency, abnormal gtpbp3zf2239/zf2239 (AB) standard conditions Fig. 7 from Chen et al., 2019
mitochondrial alanyl-tRNA aminoacylation increased occurrence, abnormal gtpbp3zf2239/zf2239 (AB) standard conditions Fig. 4 from Chen et al., 2019
whole organism mitochondrion tfam expression decreased amount, abnormal gtpbp3zf2239/zf2239 (AB) standard conditions Fig. 5 from Chen et al., 2019
whole organism respiratory chain complex I decreased amount, abnormal gtpbp3zf2239/zf2239 (AB) standard conditions Fig. 6 from Chen et al., 2019
cardiac muscle cell nucleus shape, abnormal gtpbp3zf2239/zf2239 (AB) standard conditions Fig. 9 from Chen et al., 2019
heart mitochondrion swollen, abnormal gtpbp3zf2239/zf2239 (AB) standard conditions Fig. 10 from Chen et al., 2019
heart nppa expression increased amount, abnormal gtpbp3zf2239/zf2239 (AB) standard conditions Fig. 9 from Chen et al., 2019
whole organism mitochondrion mt-cyb expression decreased amount, abnormal gtpbp3zf2239/zf2239 (AB) standard conditions Fig. 5 from Chen et al., 2019
whole organism mitochondrion sdha expression decreased amount, abnormal gtpbp3zf2239/zf2239 (AB) standard conditions Fig. 5 from Chen et al., 2019
whole organism obsolete mitochondrial respiratory chain complex IV decreased efficiency, abnormal gtpbp3zf2239/zf2239 (AB) standard conditions Fig. 7 from Chen et al., 2019
cardiac muscle cell I band increased length, abnormal gtpbp3zf2239/zf2239 (AB) standard conditions Fig. 9 from Chen et al., 2019
mitochondrial tyrosyl-tRNA aminoacylation increased occurrence, abnormal gtpbp3zf2239/zf2239 (AB) standard conditions Fig. 4 from Chen et al., 2019
heart looping disrupted, abnormal gtpbp3zf2239/zf2239; twu34Tg standard conditions Fig. 8 from Chen et al., 2019
heart contraction disrupted, abnormal gtpbp3zf2239/zf2239; twu34Tg standard conditions Fig. 8 from Chen et al., 2019
Citations