CRISPR

CRISPR2-mc4r

ID

ZDB-CRISPR-190718-2

Name

CRISPR2-mc4r

Previous Names

None

Target

mc4r (1)

Sequence

5' - GGCTGACCAGGCCGAGCGTG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
zf3069	mc4r
zf3584	mc4r

Expression

Gene expression in Wild Types + CRISPR2-mc4r

No data available

Phenotype

Phenotype resulting from CRISPR2-mc4r

No data available

Phenotype of all Fish created by or utilizing CRISPR2-mc4r

Phenotype	Fish	Conditions	Figures
whole organism increased weight, abnormal	mc4r^{zf3069/zf3069}	increased food availability	Fig. 2 from Fei et al., 2017
whole organism fatty, abnormal	mc4r^{zf3069/zf3069}	increased food availability	Fig. 2 from Fei et al., 2017
eating behavior increased frequency, abnormal	mc4r^{zf3069/zf3069}	increased food availability	Fig. 2 from Fei et al., 2017
feeding behavior behavioral quality of a process, abnormal	mc4r^{zf3069/zf3069}	increased food availability	Fig. 2 from Fei et al., 2017
glucose homeostasis decreased efficacy, abnormal	mc4r^{zf3069/zf3069}	standard conditions	Fig. 2 from Fei et al., 2017
whole organism increased mass, abnormal	mc4r^{zf3584/zf3584}	increased food availability, chemical treatment by environment: doxycycline	Fig. 3 from Fei et al., 2020
adipose tissue increased amount, abnormal	mc4r^{zf3584/zf3584}	increased food availability, chemical treatment by environment: doxycycline	Fig. 3 from Fei et al., 2020
whole organism increased length, abnormal	mc4r^{zf3584/zf3584}	increased food availability, chemical treatment by environment: doxycycline	Fig. 3 from Fei et al., 2020
adult feeding behavior increased occurrence, abnormal	mc4r^{zf3584/zf3584}	increased food availability, chemical treatment by environment: doxycycline	Fig. 3 from Fei et al., 2020
adipose tissue increased amount, abnormal	lepr^{zf3583/zf3583}; mc4r^{zf3584/zf3584}	increased food availability, chemical treatment by environment: doxycycline	Fig. 3 from Fei et al., 2020
whole organism increased mass, abnormal	lepr^{zf3583/zf3583}; mc4r^{zf3584/zf3584}	increased food availability, chemical treatment by environment: doxycycline	Fig. 3 from Fei et al., 2020
whole organism increased length, abnormal	lepr^{zf3583/zf3583}; mc4r^{zf3584/zf3584}	increased food availability, chemical treatment by environment: doxycycline	Fig. 3 from Fei et al., 2020
adult feeding behavior increased occurrence, abnormal	lepr^{zf3583/zf3583}; mc4r^{zf3584/zf3584}	increased food availability, chemical treatment by environment: doxycycline	Fig. 3 from Fei et al., 2020
brain prl expression decreased amount, abnormal	mc4r^{zf3584/zf3584}; gz26Tg/gz26Tg; gz32Tg/gz32Tg; zf3013Tg/zf3013Tg	increased food availability, chemical treatment by environment: doxycycline	Fig. 3 from Fei et al., 2020
brain asip2b expression amount, ameliorated	mc4r^{zf3584/zf3584}; gz26Tg/gz26Tg; gz32Tg/gz32Tg; zf3013Tg/zf3013Tg	increased food availability, chemical treatment by environment: doxycycline	Fig. 3 from Fei et al., 2020
blood glucose decreased amount, abnormal	mc4r^{zf3584/zf3584}; gz26Tg/gz26Tg; gz32Tg/gz32Tg; zf3013Tg/zf3013Tg	increased food availability, chemical treatment by environment: doxycycline	Fig. 3 from Fei et al., 2020
brain npy expression amount, ameliorated	mc4r^{zf3584/zf3584}; gz26Tg/gz26Tg; gz32Tg/gz32Tg; zf3013Tg/zf3013Tg	increased food availability, chemical treatment by environment: doxycycline	Fig. 3 from Fei et al., 2020
brain pomca expression amount, ameliorated	mc4r^{zf3584/zf3584}; gz26Tg/gz26Tg; gz32Tg/gz32Tg; zf3013Tg/zf3013Tg	increased food availability, chemical treatment by environment: doxycycline	Fig. 3 from Fei et al., 2020
brain oxt expression amount, ameliorated	mc4r^{zf3584/zf3584}; gz26Tg/gz26Tg; gz32Tg/gz32Tg; zf3013Tg/zf3013Tg	increased food availability, chemical treatment by environment: doxycycline	Fig. 3 from Fei et al., 2020
adult feeding behavior decreased occurrence, abnormal	mc4r^{zf3584/zf3584}; gz26Tg/gz26Tg; gz32Tg/gz32Tg; zf3013Tg/zf3013Tg	increased food availability, chemical treatment by environment: doxycycline	Fig. 3 from Fei et al., 2020
larval feeding behavior decreased occurrence, ameliorated	mc4r^{zf3584/zf3584}; gz26Tg/gz26Tg; gz32Tg/gz32Tg; zf3013Tg/zf3013Tg	increased food availability, chemical treatment by environment: doxycycline	Fig. 3 from Fei et al., 2020

Citations