CRISPR

CRISPR1-pbx4

ID

ZDB-CRISPR-190301-2

Name

CRISPR1-pbx4

Previous Names

None

Target

Sequence

5' - AGCTGCAGCGGCAGCTGGAGG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

This CRISPR contains two PAM sites "TGGAGG", which are included in the target sequence.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
scm14	pbx4

Expression

Gene expression in Wild Types + CRISPR1-pbx4

No data available

Phenotype

Phenotype resulting from CRISPR1-pbx4

No data available

Phenotype of all Fish created by or utilizing CRISPR1-pbx4

Phenotype	Fish	Conditions	Figures
heart rudiment formation decreased process quality, exacerbated	pbx4^b557/+; pbx4^scm14/+; Df(Chr01:hand2)s6/s6 (AB)	standard conditions	Fig. 3 from Farr et al., 2018
heart rudiment split bilaterally, exacerbated	pbx4^b557/+; pbx4^scm14/+; Df(Chr01:hand2)s6/s6 (AB)	standard conditions	Fig. 3 from Farr et al., 2018
heart morphogenesis decreased process quality, exacerbated	pbx4^b557/+; pbx4^scm14/+; Df(Chr01:hand2)s6/s6 (AB)	standard conditions	Fig. 3 from Farr et al., 2018
myocardium mislocalised posteriorly, abnormal	pbx4^b557/+; pbx4^scm14/+; Df(Chr01:hand2)s6/s6 (AB)	standard conditions	Fig. 3 from Farr et al., 2018

Citations