CRISPR

CRISPR1-dnaaf2

ID

ZDB-CRISPR-190103-1

Name

CRISPR1-dnaaf2

Previous Names

None

Target

dnaaf2 (1)

Sequence

5' - GGAGATCCGGCCACAGCTGG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
ja104	dnaaf2

Expression

Gene expression in Wild Types + CRISPR1-dnaaf2

No data available

Phenotype

Phenotype resulting from CRISPR1-dnaaf2

No data available

Phenotype of all Fish created by or utilizing CRISPR1-dnaaf2

Phenotype	Fish	Conditions	Figures
sperm axoneme Ab1-dnali1 labeling decreased amount, abnormal	dnaaf2^ja104/ja104 (TL)	standard conditions	Fig. 4 from Yamaguchi et al., 2018
sperm inner dynein arm decreased amount, abnormal	dnaaf2^ja104/ja104 (TL)	standard conditions	Fig. 3 from Yamaguchi et al., 2018
Kupffer's vesicle cilium movement process quality, abnormal	dnaaf2^ja104/ja104 (TL)	standard conditions	Fig. 5 from Yamaguchi et al., 2018
heart looping process quality, abnormal	dnaaf2^ja104/ja104 (TL)	standard conditions	Fig. 5 from Yamaguchi et al., 2018
flagellated sperm motility process quality, abnormal	pih1d2^ja103/ja103; dnaaf2^ja104/ja104	standard conditions	Fig. 2 from Yamaguchi et al., 2018
sperm axoneme Ab1-dnah5l labeling decreased amount, abnormal	pih1d2^ja103/ja103; dnaaf2^ja104/ja104	standard conditions	Fig. 4 from Yamaguchi et al., 2018
sperm axoneme Ab1-dnai1 labeling decreased amount, abnormal	pih1d2^ja103/ja103; dnaaf2^ja104/ja104	standard conditions	Fig. 4 from Yamaguchi et al., 2018
sperm sperm flagellum Ab1-dnah5l labeling decreased distribution, abnormal	pih1d2^ja103/ja103; dnaaf2^ja104/ja104	standard conditions	Fig. 4 from Yamaguchi et al., 2018
sperm sperm flagellum Ab1-dnah5l labeling spatial pattern, abnormal	pih1d2^ja103/ja103; dnaaf2^ja104/ja104	standard conditions	Fig. 4 from Yamaguchi et al., 2018
Kupffer's vesicle cilium immobile, abnormal	pih1d2^ja103/ja103; dnaaf2^ja104/ja104	standard conditions	Fig. 5 from Yamaguchi et al., 2018
heart looping process quality, abnormal	pih1d2^ja103/ja103; dnaaf2^ja104/ja104	standard conditions	Fig. 5 from Yamaguchi et al., 2018
sperm axonemal dynein complex composition, abnormal	pih1d2^ja103/ja103; dnaaf2^ja104/ja104	standard conditions	Fig. 3 from Yamaguchi et al., 2018
sperm distal side immobile, abnormal	pih1d2^ja103/ja103; dnaaf2^ja104/ja104	standard conditions	Fig. 2 from Yamaguchi et al., 2018
sperm axoneme Ab1-dnali1 labeling decreased amount, abnormal	pih1d2^ja103/ja103; dnaaf2^ja104/ja104	standard conditions	Fig. 4 from Yamaguchi et al., 2018
sperm cellular motility, abnormal	pih1d2^ja103/ja103; dnaaf2^ja104/ja104	standard conditions	Fig. 2 from Yamaguchi et al., 2018

Citations