- Title
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DANA elements: A family of composite, tRNA-derived short interspersed DNA elements associated with mutational activities in zebrafish (Danio rerio)
- Authors
- Izsvák, Z., Ivics, Z., Garcia-Estefania, D., Fahrenkrug, S.C., and Hackett, P.B.
- Source
- Full text @ Proc. Natl. Acad. Sci. USA
DANA is Danio-specific. Southern blot of PCR products obtained with primers Dana-1 and Dana-2 (Fig. 2) was hybridized with a DANA probe (Fig. 1). Genomic DNA samples from the following species were analyzed: zebrafish (Danio rerio) (lane 1), pearl danio (Danio albolineatus (lane 2), giant danio (Danio aequipinnatus) (lane 3), rasbora (Rasbora heteromorpha) (lane 4), common carp (Cyprinus carpio) (lane 5), white cloud mountainfish (Tanichthys albonubes) (lane 6), tiger barb (Puntius tetrazona) (lane 7), and striped loach (Acanthophthalmus kuhli) (lane 8). The phylogenetic classification of these fish species is given at the top. |
DNA polymorphisms generated by DANA-specific primers in PCR. 32P-labeled PCR amplification products were separated in polyacrylamide gels. (A) Effect of the specificity and combination of PCR primers on the number of DNA fragments amplified from Singapore zebrafish DNA. Lanes: 1, Dana-1 primer only; 2, Dana-1 supplemented with two TdrI-specific primers, TdrA and FTC-12. (B) Polymorphic patterns generated by DANA-PCR using the same primer combination as in lane 2 of A, on DNA samples from different zebrafish populations. Lanes: 1, Hong Kong; 2, Indonesia; 3, Singapore; 4, wild-type 1 (United States); 5, wild-type 2 (United States); 6, “gold” zebrafish; 7, clonal line C-32; 8, “leopard”/C-32. |