Locomotion-based prepulse inhibition and modulation by MK-801: A. Locomotion trace showing the response of all larvae (6dpf) after within-subject presentation of eight 200 Hz 20 ms vibrations (two vibrations per motor step, randomised order). Data are presented as the average locomotion (mm) for each pulse intensity. B. Response magnitude of responder larvae. Data are presented as the average locomotion (mm) for each pulse intensity 100 ms after the startle stimulus. C. Response probability of all larvae. Data are presented as the average response probability per trial 100 ms after the startle stimulus. D. Diagram to show the finalised PPI assay procedure. E. 300 ms ISI raw locomotion data. Data are presented as the mean distance travelled (mm) by all larvae in startle pulse only (blue) and prepulse/startle (purple) trials. Arrows indicate time points where the prepulse (blue) and startle (purple) stimuli are delivered on relevant trials. F. Percentage PPI for responder larvae at ISIs of 100 – 1000 ms (for PPI calculation, see Methods). G. Response magnitude of responder larvae in startle alone and prepulse/startle trials across 100-1000 ms ISIs. Data are presented as average locomotion (mm) 100 ms after the startle stimulus. H. Response probability of all larvae across 100-1000 ms ISIs. Data are presented as average response probability per trial, 100 msec after the startle stimulus. I. Percentage PPI for responder larvae treated with MK-801 or a DMSO-control solution. J. Response magnitude of responder larvae treated with MK-801 or a DMSO-control solution in startle alone and prepulse/startle trials. Data are presented as average locomotion (mm) 100 ms after the startle stimulus. K. Response probability of all larvae treated with MK-801 or a DMSO-control solution in startle alone and prepulse/startle trials. Data are presented as average response probability per trial, 100 ms after the startle stimulus. For all N-numbers, see Table 1 in Methods section. * = p<0.05, ** = p<0.01, *** = p<0.001, **** = p<0.0001.