- Title
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Ryanodine receptor-active non-dioxin-like polychlorinated biphenyls cause neurobehavioral deficits in larval zebrafish
- Authors
- Yaghoobi, B., Miller, G.W., Holland, E.B., Li, X., Harvey, D., Li, S., Lehmler, H.J., Pessah, I.N., Lein, P.J.
- Source
- Full text @ Front Toxicol
PCB induced [3H]-labeled ryanodine activation and binding in adult zebrafish skeletal muscle. |
PCB exposures did not significantly decrease viability (congeners listed in order of increasing RYR binding affinity). Larvae were observed for overt malformations or mortalities at 1, 3, 4, and 5 days post-fertilization (dpf). Data are presented as mean percentage (malformed and dead) or mean percentage (viable) ± SEM from ≥3 separate spawns. The total larvae per exposure (Vehicle (Veh), 0.1, 0.3, 1.0, 3.0, and 10 µM) was the following: PCB 66: 32, 60, 61, 63, 60, and 60; PCB 28: 23, 45, 44, 48, 48, and 47; PCB 153: 28, 58, 58, 58, 60, and 60; PCB 138: 28, 59, 61, 63, 59, and 59; PCB 84: 32, 56, 56, 57, 52, and 57; PCB 95: 61, 85, 115, 111, 113, and 119. |
PCB exposure (10 µM) significantly alters larval photomotor behavior at 3 and 5 days post-fertilization (dpf) compared to the vehicle (DMSO) control in a RYR-dependent pattern. Examples of photomotor behavior at 3, 4, and 5 dpf from embryos exposed to only vehicle (DMSO, black lines) or 10 µM (grey line) of PCB 66 (low RYR-activity), PCB 153 (mid-level RYR-activity) and PCB 95 (high RYR-activity). The 35-min behavioral testing period was divided into five epochs: 0–5 min = acclimation period, 5–10 min = Light 1, 11–15 = Dark 1, 16–20 min = Light 2 and 21–35 min = Dark 2. Shaded areas correspond to dark periods. Data are presented as mean ± SEM ( |
PCB levels in pooled whole fish at 5 dpf exposed to PCB starting at 6 hpf. Each point represents the average body burden in ng per pooled sample of 36–51 larvae collected at 5 dpf. Wet weight per pool is approximately 1 mg, allowing for the extrapolation to mg/kg. |
Congener rankings. Rankings were assigned to each congener based on the average ranking from three assays: RYR binding, behavior and body burden. |