Biodistribution of the rhodamine labeled peptides (Rho-P1 to Rho-P6) in zebrafish embryos. A,A´) Rho-P1, B,B´) Rho-P2, C,C´)Rho-P3, D,D´) Rho-P4, E,E´) Rho-P5, and f,F,F´) Rho-P6 were intravenously injected into 3 dpf Tg(kdrl:EGFP)s843Tg zebrafish embryos and 3D images from the head (A–F) and trunk (A´–F´) regions were acquired every hour for 19 h (n = 3 embryos for each peptide). f) A single focal plane in the brain region was selected to highlight the distribution of Rho-P6 along the radial glia fiber. Images shown are from 1 h-post-injection. Peptide distribution and blood vessels are shown in magenta and green, respectively. The fluorescence microscopy images are superimposed on bright-field images (grey) from a selected single plane. Lateral views of the embryos are oriented anterior left with dorsal up. a, anterior; p, posterior; d, dorsal; v, ventral; ca, caudal artery; cvp, caudal vein plexus; s, spinal cord. Scale bar: 50 µm.
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